Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • MacDonald, D.W.; Cove, D.J.
    Studies on temperature-sensitive mutants affecting the assimilatory nitrate reductase of Aspergillus nidulans (1974), Eur. J. Biochem., 47, 107-110.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.3 additional information studies of temperature-sensitive mutations, niaD gene: mutation leads to loss of a 4.5-S cytochrome-c reductase activity, which is a subunit of nitrate reductase. It is suggested that neither the product of the cnxE nor the cnyF genes form part of the nitrate reductase molecule, but some catalytic role in cofactor formation, niaD and cnxH seem to be structural genes Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.3 Molybdenum molybdenum containing cofactor Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH Aspergillus nidulans
-
 nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH Aspergillus nidulans biA-1
-
 nitrite + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Aspergillus nidulans
-
 biA-1
-
1.7.1.3 Aspergillus nidulans biA-1
-
 biA-1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.3 0.004 0.074 various temperature-sensitive mutants Aspergillus nidulans
1.7.1.3 0.074
-
 wild type Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH
-
 Aspergillus nidulans nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH some mutants use hypoxanthine as nitrogen source, 4.5-S cytochrome-c reductase activity Aspergillus nidulans nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH
-
 Aspergillus nidulans biA-1 nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH some mutants use hypoxanthine as nitrogen source, 4.5-S cytochrome-c reductase activity Aspergillus nidulans biA-1 nitrite + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 multimer
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.3 25
-
 wild-type half-life: 120 min Aspergillus nidulans
1.7.1.3 30
-
 wild-type half-life: 66 min Aspergillus nidulans
1.7.1.3 35
-
 wild-type half-life: 18.8 min, various temperature sensitive mutant strains: 3.8-18.7 min Aspergillus nidulans
1.7.1.3 37
-
 wild-type half-life: 14.5 min Aspergillus nidulans