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Literature summary extracted from

  • Xing, R.; Whitman, W.B.
    Purification and characterization of the oxygen-sensitive acetohydroxy acid synthase from the archaebacterium Methanococcus aeolicus (1994), J. Bacteriol., 176, 1207-1213.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.2.1.6 NH4+ 200 mM, 1.6fold stimulation Methanococcus aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 6.8
-
 pyruvate
-
 Methanococcus aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ Mn2+ or Mg2+ required Methanococcus aeolicus
2.2.1.6 Mg2+ Km: 0.3 mM MgCl2 Methanococcus aeolicus
2.2.1.6 Mn2+ Mn2+ or Mg2+ required Methanococcus aeolicus
2.2.1.6 Mn2+ Km: 0.016 mM MnCl2 Methanococcus aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Methanococcus aeolicus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.2.1.6 5% O2, half-life: 35 min Methanococcus aeolicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6
-
 Methanococcus aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Methanococcus aeolicus 2-acetolactate + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.6
-
-
 Methanococcus aeolicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD Km: 0.0004 mM Methanococcus aeolicus
2.2.1.6 FMN stimulates, Km: 0.0013 mM Methanococcus aeolicus
2.2.1.6 riboflavin plus 10 mM phosphate, stimulates Methanococcus aeolicus
2.2.1.6 thiamine diphosphate required as cofactor Methanococcus aeolicus
2.2.1.6 thiamine diphosphate Km: 0.0016 Methanococcus aeolicus