Any feedback?
Literature summary extracted from
- Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli (1989), Eur. J. Biochem., 185, 433-439.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.2.1.6 | expression in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.2.1.6 | 3.93 | - |
pyruvate | - |
Saccharomyces cerevisiae |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.2.1.6 | Mg2+ | required | Saccharomyces cerevisiae | |
| 2.2.1.6 | Mg2+ | Km: 0.28 mM | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.6 | 75000 | - |
2 * 75000, SDS-PAGE | Saccharomyces cerevisiae |
| 2.2.1.6 | 150000 | - |
- |
Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.2.1.6 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.2.1.6 | - |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.6 | additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.2.1.6 | pyruvate | - |
Saccharomyces cerevisiae | 2-acetolactate + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.2.1.6 | dimer | 2 * 75000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.6 | 6.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.6 | 6 | 7.5 | pH 6.0: about 25% of maximal activity, pH 7.5: about 65% of maximal activity | Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.2.1.6 | FAD | required | Saccharomyces cerevisiae | |
| 2.2.1.6 | FAD | Km: 0.0003 mM | Saccharomyces cerevisiae | |
| 2.2.1.6 | thiamine diphosphate | required as cofactor | Saccharomyces cerevisiae | |
| 2.2.1.6 | thiamine diphosphate | Km: 0.11 mM | Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
