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Literature summary extracted from

  • Grimminger, H.; Umbarger, H.E.
    Acetohydroxy acid synthase I of Escherichia coli: purification and properties (1979), J. Bacteriol., 137, 846-853.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 Val isoenzyme I and III inhibited Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Escherichia coli
2.2.1.6 Mg2+ Km: 0.06 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 60000
-
 2 * 60000, SDS-PAGE Escherichia coli
2.2.1.6 130000
-
 sucrose density gradient sedimentation, glycerol density gradient sedimentation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Escherichia coli
-
 isoenzyme I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 isoenzyme I Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.2.1.6 4°C, stable for several days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Escherichia coli 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 dimer 2 * 60000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.5
-
 isoenzyme I Escherichia coli
2.2.1.6 8.5
-
 isoenzyme III Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD required Escherichia coli
2.2.1.6 FAD Km: 0.0003 mM Escherichia coli
2.2.1.6 thiamine diphosphate required as cofactor Escherichia coli
2.2.1.6 thiamine diphosphate Km: 0.0012 mM Escherichia coli