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Literature summary extracted from

  • Spence, E.L.; Kawamukai, M.; Sanvoisin, J.; Braven, H.; Bugg, T.D.
    Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): sequence analysis and biochemical properties of a third family of extradiol dioxygenases (1996), J. Bacteriol., 178, 5249-5256.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.16 activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable Cupriavidus necator
1.13.11.16 activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.16 diethyl dicarbonate
-
 Cupriavidus necator
1.13.11.16 diethyl dicarbonate
-
 Escherichia coli
1.13.11.16 DTNB
-
 Cupriavidus necator
1.13.11.16 DTNB
-
 Escherichia coli
1.13.11.16 p-hydroxymercuribenzoate
-
 Cupriavidus necator
1.13.11.16 p-hydroxymercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.16 0.0071
-
 3-(2,3-dihydroxyphenyl)propionate
-
 Cupriavidus necator
1.13.11.16 0.014
-
 2,3-dihydroxycinnamic acid
-
 Cupriavidus necator
1.13.11.16 0.036
-
 2,3-dihydroxycinnamic acid
-
 Escherichia coli
1.13.11.16 0.037
-
 methyl-2,3-dihydroxyphenylpropionate
-
 Escherichia coli
1.13.11.16 0.059
-
 3-ethylcatechol
-
 Cupriavidus necator
1.13.11.16 0.094
-
 3-phenethylcatechol
-
 Escherichia coli
1.13.11.16 0.13
-
 3-methylcatechol
-
 Cupriavidus necator
1.13.11.16 0.154
-
 3-propylcatechol
-
 Escherichia coli
1.13.11.16 0.185
-
 3-ethylcatechol
-
 Escherichia coli
1.13.11.16 0.3
-
 2,3-dihydroxyphenoxyacetic acid
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.16 3-(2,3-dihydroxyphenyl)propionate + O2 Cupriavidus necator
-
 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?
1.13.11.16 3-(2,3-dihydroxyphenyl)propionate + O2 Escherichia coli
-
 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.16 Cupriavidus necator
-
-
-
1.13.11.16 Escherichia coli P0ABR9 gene mhpB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.16
-
 Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.16 4.3
-
 2,3-dihydroxyphenylproponic acid Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.16 2,3-dihydroxycinnamic acid + O2
-
 Cupriavidus necator 2-hydroxy-6-oxonona-2,4,7-trienoate
-
 ?
1.13.11.16 2,3-dihydroxycinnamic acid + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-2,4,7-trienoate
-
 ?
1.13.11.16 2,3-dihydroxyphenoxyacetic acid + O2
-
 Escherichia coli 2-hydroxyhexa-2,4-dienoic acid-6-carboxymethylester
-
 ?
1.13.11.16 2,3-dihydroxyphenylpropionic acid + O2
-
 Cupriavidus necator 2-hydroxy-6-oxonona-dienoate
-
 ?
1.13.11.16 2,3-dihydroxyphenylpropionic acid + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-dienoate
-
 ?
1.13.11.16 3-(2,3-dihydroxyphenyl)propionate + O2
-
 Cupriavidus necator 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?
1.13.11.16 3-(2,3-dihydroxyphenyl)propionate + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate
-
 ?
1.13.11.16 3-ethylcatechol + O2
-
 Cupriavidus necator 2-hydroxy-6-oxoocta-2,4-dienoate
-
 ?
1.13.11.16 3-ethylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxoocta-2,4-dienoate
-
 ?
1.13.11.16 3-methylcatechol + O2
-
 Cupriavidus necator 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.16 3-methylcatechol + O2 meta-ring cleavage Escherichia coli 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.16 3-phenethylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxo-8-phenylocta-2,4-dienoate
-
 ?
1.13.11.16 3-propylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxonona-2,4-dienoate
-
 ?
1.13.11.16 methyl-(2,3-dihydroxyphenyl)propionate + O2
-
 Escherichia coli (2Z,4E)-2-hydroxy-9-methoxy-6,9-dioxonona-2,4-dienoic acid
-
 ?