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Literature summary extracted from

  • Kunapuli, S.P.; Vaidyanathan, C.S.
    Indole oxygenase from the leaves of Tecoma stans (1982), Plant Sci. Lett., 24, 183-188.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.13.11.17 dialysis results in complete loss of activity Tecoma stans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.17 Atebrin no effect Tecoma stans
1.13.11.17 azide
-
Tecoma stans
1.13.11.17 Cu2+ 1 mM, CuSO4, 25% inhibition Tecoma stans
1.13.11.17 cyanide
-
Tecoma stans
1.13.11.17 diethyldithiocarbamate
-
Tecoma stans
1.13.11.17 Dithionite
-
Tecoma stans
1.13.11.17 Fe2+ 0.5 mM, 35% inhibition Tecoma stans
1.13.11.17 Salicylaldoxime
-
Tecoma stans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.17 Tecoma stans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.17
-
Tecoma stans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.17 leaf
-
Tecoma stans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.17 0.0157
-
disappearance of 1 nmol indole in 1 min Tecoma stans

Storage Stability

EC Number Storage Stability Organism
1.13.11.17 -20°C, 18 h without appreciable loss of activity, complete loss of activity after 48 h Tecoma stans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.17 5-bromoindole + O2
-
Tecoma stans 5-bromo-2-formylaminobenzaldehyde
-
?
1.13.11.17 5-hydroxyindole + O2
-
Tecoma stans 2-formylamino-5-hydroxybenzaldehyde
-
?
1.13.11.17 5-methylindole + O2
-
Tecoma stans 2-formylamino-5-methylbenzaldehyde
-
?
1.13.11.17 indole + O2 conversion of indole to anthranilic acid Tecoma stans anthranilic acid
-
?
1.13.11.17 o-aminobenzaldehyde + O2
-
Tecoma stans anthranilic acid
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.17 30
-
-
Tecoma stans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.17 5.2
-
-
Tecoma stans

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.17 FAD not a flavoprotein Tecoma stans