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Literature summary extracted from

  • Divakar, N.G.; Subramanian, V.; Sugumaran, M.; Vaidyanathan, C.S.
    Indole oxygenase from the leaves of Jasminum grandiflorum (1979), Plant Sci. Lett., 15, 177-181.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.13.11.17 dialysis results in complete loss of activity Jasminum grandiflorum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.17 2-mercaptoethanol 0.0005 mM, 64.5% inhibition Jasminum grandiflorum
1.13.11.17 8-hydroxyquinoline 0.001 mM, 48% inhibition Jasminum grandiflorum
1.13.11.17 diethyldithiocarbamate 0.001 mM, 74% inhibition Jasminum grandiflorum
1.13.11.17 dithiothreitol 0.0005 mM, 97% inhibition Jasminum grandiflorum
1.13.11.17 Fe2+ 0.0005 mM, 55% inhibition Jasminum grandiflorum
1.13.11.17 GSH 0.0005 mM, 94% inhibition Jasminum grandiflorum
1.13.11.17 iodoacetate 0.0005 mM, 19% inhibition Jasminum grandiflorum
1.13.11.17 NEM 0.0005 mM, 87.5% inhibition Jasminum grandiflorum
1.13.11.17 Neocuproine 0.001 mM, 92% inhibition Jasminum grandiflorum
1.13.11.17 PCMB 0.0005 mM, 98.5% inhibition Jasminum grandiflorum
1.13.11.17 Salicylaldoxime 0.001 mM, 61% inhibition Jasminum grandiflorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.17 Cu2+ reconstitution of enzyme activity after dialysis by FAD and Cu2+ Jasminum grandiflorum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.17 Jasminum grandiflorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.17
-
 Jasminum grandiflorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.17 leaf
-
 Jasminum grandiflorum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.17 0.00575
-
-
 Jasminum grandiflorum

Storage Stability

EC Number Storage Stability Organism
1.13.11.17 -20°C, 20 d, no appreciable loss of activity Jasminum grandiflorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.17 5-bromoindole + O2
-
 Jasminum grandiflorum 5-bromo-2-formylaminobenzaldehyde
-
 ?
1.13.11.17 5-hydroxyindole + O2
-
 Jasminum grandiflorum 2-formylamino-5-hydroxybenzaldehyde
-
 ?
1.13.11.17 indole + O2 conversion of indole to anthranilic acid Jasminum grandiflorum anthranilic acid
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.17 30
-
-
 Jasminum grandiflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.17 4.8
-
-
 Jasminum grandiflorum

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.17 FAD reconstitution of enzyme activity after dialysis by FAD and Cu2+ Jasminum grandiflorum