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Literature summary extracted from

  • Yagi, T.; Kimura, K.; Daidoji, H.; Sakai, F.; Tamura, S.; Inokuchi, H.
    Properties of purified hydrogenase from the particulate fraction of Desulfovibrio vulgaris, Miyazaki (1976), J. Biochem., 79, 661-671.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.2.1 CO
-
 Desulfovibrio vulgaris
1.12.2.1 EDTA 10 mM, 10% inhibition Desulfovibrio vulgaris
1.12.2.1 H2O2
-
 Desulfovibrio vulgaris
1.12.2.1 NEM 2 mM; 60% inhibition Desulfovibrio vulgaris
1.12.2.1 p-chloromercuribenzene sulfonate 1 mM; 87% inhibition Desulfovibrio vulgaris
1.12.2.1 PCMB 0.17 mM, 35% inhibition Desulfovibrio vulgaris
1.12.2.1 SDS 0.1%, 86-99% inactivation Desulfovibrio vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.2.1 particle-bound
-
 Desulfovibrio vulgaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.2.1 Iron iron-sulfur protein, 7-9 iron atoms and 7-8 labile sulfide ions Desulfovibrio vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.2.1 28000
-
 1 * 28000 + 1 * 59000, SDS-PAGE Desulfovibrio vulgaris
1.12.2.1 59000
-
 1 * 28000 + 1 * 59000, SDS-PAGE Desulfovibrio vulgaris
1.12.2.1 89000
-
 low-speed equilibrium sedimentation Desulfovibrio vulgaris

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.12.2.1 urea 9 M, 24 h, room temperature, stable Desulfovibrio vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.12.2.1 Desulfovibrio vulgaris
-
 Miyazaki
-
1.12.2.1 Desulfovibrio vulgaris Miyazaki
-
 Miyazaki
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.2.1
-
 Desulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.2.1 H+ + reduced methyl viologen
-
 Desulfovibrio vulgaris H2 + methyl viologen
-
 ?
1.12.2.1 H+ + reduced methyl viologen
-
 Desulfovibrio vulgaris Miyazaki H2 + methyl viologen
-
 ?
1.12.2.1 H2 + ferricytochrome c3
-
 Desulfovibrio vulgaris H+ + ferrocytochrome c3
-
 ?
1.12.2.1 H2 + ferricytochrome c3
-
 Desulfovibrio vulgaris Miyazaki H+ + ferrocytochrome c3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.2.1 dimer 1 * 28000 + 1 * 59000, SDS-PAGE Desulfovibrio vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.2.1 cytochrome c3
-
 Desulfovibrio vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.12.2.1 additional information
-
 additional information Ki for CO is 8.9 Torr Desulfovibrio vulgaris