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Literature summary extracted from

  • Littlejohn, T.K.; Takikawa, O.; Skylas, D.; Jamie, J.F.; Walker, M.J.; Truscott, R.J.W.
    Expression and purification of recombinant human indoleamine 2,3-dioxygenase (2000), Protein Expr. Purif., 19, 22-29.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.52 ascorbic acid native and recombinant enzyme require methylene blue and ascorbic acid for activity Homo sapiens
1.13.11.52 methylene blue native and recombinant enzyme require methylene blue and ascorbic acid for activity Homo sapiens

Application

EC Number Application Comment Organism
1.13.11.52 medicine first and rate-limiting enzyme in tryptophan metabolism, plays a role in pathogenesis of many diseases Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.52 expression in Escherichia coli E538, expressed with His-Tag Homo sapiens
1.13.11.52 expression in Escherichia coli EC538 as a fusion protein to a hexahistidyl tag Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.02
-
 L-tryptophan
-
 Homo sapiens
1.13.11.52 0.02
-
 L-Trp recombinant enzyme Homo sapiens
1.13.11.52 0.44
-
 5-hydroxy-L-tryptophan
-
 Homo sapiens
1.13.11.52 0.44
-
 5-hydroxy-L-tryptophan recombinant enzyme Homo sapiens
1.13.11.52 5
-
 D-tryptophan
-
 Homo sapiens
1.13.11.52 5
-
 D-Trp recombinant enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.52 45440
-
 ESI mass spectrometry Homo sapiens
1.13.11.52 46980
-
 ESI mass spectrometry Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52 phosphocellulose and Ni-NTA-agarose affinity chromatography Homo sapiens
1.13.11.52 recombinant enzyme, fusion protein to a hexahistidyl tag Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.52 2.48
-
 kynurenine, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.2 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 2.58
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 5-hydroxy-L-tryptophan + O2
-
 Homo sapiens N-formyl-5-hydroxy-L-kynurenine
-
 ?
1.13.11.52 D-Trp + O2
-
 Homo sapiens N-formyl-D-kynurenine
-
 ?
1.13.11.52 D-tryptophan + O2
-
 Homo sapiens N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-5-hydroxytryptophan + O2
-
 Homo sapiens ?
-
 ?
1.13.11.52 L-Trp + O2
-
 Homo sapiens L-formylkynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Homo sapiens N-formyl-L-kynurenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.52 IDO
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.52 0.065
-
 5-hydroxy-L-tryptophan
-
 Homo sapiens
1.13.11.52 1.55
-
 L-Trp
-
 Homo sapiens
1.13.11.52 2.07
-
 D-Trp
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.52 heme
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.52 Homo sapiens isoelectric focusing
-
 7.09