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Literature summary extracted from

  • Matsumura, M.; Osada, K.; Aiba, S.
    L-Tryptophan 2,3-dioxygenase of a moderate thermophile, Bacillus brevis. Purification, properties and a substrate-mediated stabilization of the quaternary structure (1984), Biochim. Biophys. Acta, 786, 9-17.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.52 guanidine hydrochloride, 30°C, 30 min, about 15% loss of activity at 0.5 M, about 30% loss of activity at 1 mM, about 60% loss of activity at 2 M, complete loss of activity at 3 mM Brevibacillus brevis
1.13.11.52 urea, 30°C, 30 min, stable at 2.5 M, about 30% loss of activity at 5.0 M, about 60% loss of activity at 7.5 M Brevibacillus brevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 CN- 0.4 mM, 86% inhibition Brevibacillus brevis
1.13.11.52 CO 20% with 80% air, 78% inhibition Brevibacillus brevis
1.13.11.52 D-Trp no inhibition Brevibacillus brevis
1.13.11.52 DL-5-hydroxytryptophan 1 mM, 55% inhibition Brevibacillus brevis
1.13.11.52 indole 1 mM, 44% inhibition Brevibacillus brevis
1.13.11.52 Indolepropionic acid 1 mM, 31% inhibition Brevibacillus brevis
1.13.11.52 K3Fe(CN)6 1 mM, complete inhibition Brevibacillus brevis
1.13.11.52 N3- 2 mM, 22% inhibition Brevibacillus brevis
1.13.11.52 NH2OH 2 mM, 54% inhibition Brevibacillus brevis
1.13.11.52 tryptamine 1 mM, 23% inhibition Brevibacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.044
-
 O2
-
 Brevibacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.52 27000
-
 4 * 27000, SDS-PAGE Brevibacillus brevis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Brevibacillus brevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52
-
 Brevibacillus brevis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.52 3.85
-
-
 Brevibacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 DL-5-fluoro-methyltryptophan + O2 35% of the activity with L-Trp Brevibacillus brevis ?
-
 ?
1.13.11.52 DL-5-methyltryptophan + O2 90% of the activity with L-Trp Brevibacillus brevis ?
-
 ?
1.13.11.52 DL-6-fluorotryptophan + O2 90% of the activity with L-Trp Brevibacillus brevis ?
-
 ?
1.13.11.52 DL-6-methyltryptophan 98% of the activity with L-Trp Brevibacillus brevis ?
-
 ?
1.13.11.52 L-Trp + O2
-
 Brevibacillus brevis L-formylkynurenine
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.52 tetramer 4 * 27000, SDS-PAGE Brevibacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.52 55
-
-
 Brevibacillus brevis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.52 additional information
-
 L-Trp stabilizes against heat inactivation Brevibacillus brevis
1.13.11.52 70
-
 30 min, stable in presence of 5 mM L-Trp Brevibacillus brevis
1.13.11.52 75
-
 30 min, about 5% loss of activity in presence of 5 mM L-Trp Brevibacillus brevis
1.13.11.52 80
-
 30 min, about 35% loss of activity in presence of 5 mM L-Trp Brevibacillus brevis
1.13.11.52 85
-
 30 min, complete loss of activity in presence of 5 mM L-Trp Brevibacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.52 7.5
-
-
 Brevibacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.52 heme contains 1 mol or more of heme per mol of enzyme Brevibacillus brevis