BRENDA - Enzyme Database

Mechanism of action of putrescine oxidase. Binding characteristics of the active site of putrescine oxidase from Micrococcus rubens

Swain, W.F.; Desa, R.J.; Biochim. Biophys. Acta 429, 331-341 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.10
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
1,10-Diaminodecane
-
Kocuria rosea
1.4.3.10
1,12-diaminododecane
-
Kocuria rosea
1.4.3.10
1,3-Diaminopropane
-
Kocuria rosea
1.4.3.10
1,6-diaminohexane
-
Kocuria rosea
1.4.3.10
1,7-Diaminoheptane
-
Kocuria rosea
1.4.3.10
1,8-diaminooctane
-
Kocuria rosea
1.4.3.10
1-aminoethanol
-
Kocuria rosea
1.4.3.10
2-Aminoethanol
-
Kocuria rosea
1.4.3.10
3-Amino-1-propanol
-
Kocuria rosea
1.4.3.10
4-amino-1-butanoic acid
-
Kocuria rosea
1.4.3.10
5-amino-1-pentanoic acid
-
Kocuria rosea
1.4.3.10
5-amino-1-pentanol
-
Kocuria rosea
1.4.3.10
6-amino-1-hexanoic acid
-
Kocuria rosea
1.4.3.10
6-amino-1-hexanol
-
Kocuria rosea
1.4.3.10
8-amino-1-octanoic acid
-
Kocuria rosea
1.4.3.10
agmatine
-
Kocuria rosea
1.4.3.10
allylamine
-
Kocuria rosea
1.4.3.10
ammonium
-
Kocuria rosea
1.4.3.10
benzylamine
-
Kocuria rosea
1.4.3.10
Butylamine
-
Kocuria rosea
1.4.3.10
dodecylamine
-
Kocuria rosea
1.4.3.10
ethylamine
-
Kocuria rosea
1.4.3.10
ethylenediamine
-
Kocuria rosea
1.4.3.10
heptylamine
-
Kocuria rosea
1.4.3.10
Hexylamine
-
Kocuria rosea
1.4.3.10
methylamine
-
Kocuria rosea
1.4.3.10
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
pentylamine
-
Kocuria rosea
1.4.3.10
Phenylethylamine
-
Kocuria rosea
1.4.3.10
Propylamine
-
Kocuria rosea
1.4.3.10
tyramine
-
Kocuria rosea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.10
Kocuria rosea
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.4.3.10
putrescine + O2 + H2O = 4-aminobutanal + NH3 + H2O2
mechanism
Kocuria rosea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.10
1,6-diaminohexane + O2 + H2O
1% of the activity with putrescine
395447
Kocuria rosea
?
-
-
-
?
1.4.3.10
cadaverine + O2 + H2O
7% of the activity with putrescine
395447
Kocuria rosea
5-aminopentanal + NH3 + H2O2
-
-
-
?
1.4.3.10
putrescine + O2 + H2O
-
395447
Kocuria rosea
4-aminobutanal + NH3 + H2O2
-
-
-
?
1.4.3.10
spermidine + O2 + H2O
10% of the activity with putrescine
395447
Kocuria rosea
4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.10
0.002
-
1,12-diaminododecane
-
Kocuria rosea
1.4.3.10
0.003
-
1,10-Diaminodecane
-
Kocuria rosea
1.4.3.10
0.0032
-
1,8-diaminooctane
-
Kocuria rosea
1.4.3.10
0.0082
-
1,7-Diaminoheptane
-
Kocuria rosea
1.4.3.10
0.0082
-
dodecylamine
-
Kocuria rosea
1.4.3.10
0.013
-
allylamine
-
Kocuria rosea
1.4.3.10
0.014
-
heptylamine
-
Kocuria rosea
1.4.3.10
0.023
-
1,6-diaminohexane
-
Kocuria rosea
1.4.3.10
0.036
-
agmatine
-
Kocuria rosea
1.4.3.10
0.039
-
Hexylamine
-
Kocuria rosea
1.4.3.10
0.039
-
tyramine
-
Kocuria rosea
1.4.3.10
0.047
-
benzylamine
-
Kocuria rosea
1.4.3.10
0.087
-
Phenylethylamine
-
Kocuria rosea
1.4.3.10
0.088
-
5-amino-1-pentanol
-
Kocuria rosea
1.4.3.10
0.12
-
1,3-Diaminopropane
-
Kocuria rosea
1.4.3.10
0.14
-
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
0.19
-
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
0.24
-
pentylamine
-
Kocuria rosea
1.4.3.10
0.31
-
6-amino-1-hexanol
-
Kocuria rosea
1.4.3.10
0.46
-
Butylamine
-
Kocuria rosea
1.4.3.10
0.5
-
3-Amino-1-propanol
-
Kocuria rosea
1.4.3.10
0.62
-
Propylamine
-
Kocuria rosea
1.4.3.10
0.67
-
3-Amino-1-propanol
-
Kocuria rosea
1.4.3.10
0.95
-
ethylenediamine
-
Kocuria rosea
1.4.3.10
2.3
-
ethylamine
-
Kocuria rosea
1.4.3.10
3
-
2-Aminoethanol
-
Kocuria rosea
1.4.3.10
3.4
-
methylamine
-
Kocuria rosea
1.4.3.10
20
-
ammonium
-
Kocuria rosea
1.4.3.10
59
-
5-amino-1-pentanoic acid
-
Kocuria rosea
1.4.3.10
160
-
8-amino-1-octanoic acid
-
Kocuria rosea
1.4.3.10
300
-
6-amino-1-hexanoic acid
-
Kocuria rosea
1.4.3.10
440
-
4-amino-1-butanoic acid
-
Kocuria rosea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.10
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
1,10-Diaminodecane
-
Kocuria rosea
1.4.3.10
1,12-diaminododecane
-
Kocuria rosea
1.4.3.10
1,3-Diaminopropane
-
Kocuria rosea
1.4.3.10
1,6-diaminohexane
-
Kocuria rosea
1.4.3.10
1,7-Diaminoheptane
-
Kocuria rosea
1.4.3.10
1,8-diaminooctane
-
Kocuria rosea
1.4.3.10
1-aminoethanol
-
Kocuria rosea
1.4.3.10
2-Aminoethanol
-
Kocuria rosea
1.4.3.10
3-Amino-1-propanol
-
Kocuria rosea
1.4.3.10
4-amino-1-butanoic acid
-
Kocuria rosea
1.4.3.10
5-amino-1-pentanoic acid
-
Kocuria rosea
1.4.3.10
5-amino-1-pentanol
-
Kocuria rosea
1.4.3.10
6-amino-1-hexanoic acid
-
Kocuria rosea
1.4.3.10
6-amino-1-hexanol
-
Kocuria rosea
1.4.3.10
8-amino-1-octanoic acid
-
Kocuria rosea
1.4.3.10
agmatine
-
Kocuria rosea
1.4.3.10
allylamine
-
Kocuria rosea
1.4.3.10
ammonium
-
Kocuria rosea
1.4.3.10
benzylamine
-
Kocuria rosea
1.4.3.10
Butylamine
-
Kocuria rosea
1.4.3.10
dodecylamine
-
Kocuria rosea
1.4.3.10
ethylamine
-
Kocuria rosea
1.4.3.10
ethylenediamine
-
Kocuria rosea
1.4.3.10
heptylamine
-
Kocuria rosea
1.4.3.10
Hexylamine
-
Kocuria rosea
1.4.3.10
methylamine
-
Kocuria rosea
1.4.3.10
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
pentylamine
-
Kocuria rosea
1.4.3.10
Phenylethylamine
-
Kocuria rosea
1.4.3.10
Propylamine
-
Kocuria rosea
1.4.3.10
tyramine
-
Kocuria rosea
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.10
0.002
-
1,12-diaminododecane
-
Kocuria rosea
1.4.3.10
0.003
-
1,10-Diaminodecane
-
Kocuria rosea
1.4.3.10
0.0032
-
1,8-diaminooctane
-
Kocuria rosea
1.4.3.10
0.0082
-
1,7-Diaminoheptane
-
Kocuria rosea
1.4.3.10
0.0082
-
dodecylamine
-
Kocuria rosea
1.4.3.10
0.013
-
allylamine
-
Kocuria rosea
1.4.3.10
0.014
-
heptylamine
-
Kocuria rosea
1.4.3.10
0.023
-
1,6-diaminohexane
-
Kocuria rosea
1.4.3.10
0.036
-
agmatine
-
Kocuria rosea
1.4.3.10
0.039
-
Hexylamine
-
Kocuria rosea
1.4.3.10
0.039
-
tyramine
-
Kocuria rosea
1.4.3.10
0.047
-
benzylamine
-
Kocuria rosea
1.4.3.10
0.087
-
Phenylethylamine
-
Kocuria rosea
1.4.3.10
0.088
-
5-amino-1-pentanol
-
Kocuria rosea
1.4.3.10
0.12
-
1,3-Diaminopropane
-
Kocuria rosea
1.4.3.10
0.14
-
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
0.19
-
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1.4.3.10
0.24
-
pentylamine
-
Kocuria rosea
1.4.3.10
0.31
-
6-amino-1-hexanol
-
Kocuria rosea
1.4.3.10
0.46
-
Butylamine
-
Kocuria rosea
1.4.3.10
0.5
-
3-Amino-1-propanol
-
Kocuria rosea
1.4.3.10
0.62
-
Propylamine
-
Kocuria rosea
1.4.3.10
0.67
-
3-Amino-1-propanol
-
Kocuria rosea
1.4.3.10
0.95
-
ethylenediamine
-
Kocuria rosea
1.4.3.10
2.3
-
ethylamine
-
Kocuria rosea
1.4.3.10
3
-
2-Aminoethanol
-
Kocuria rosea
1.4.3.10
3.4
-
methylamine
-
Kocuria rosea
1.4.3.10
20
-
ammonium
-
Kocuria rosea
1.4.3.10
59
-
5-amino-1-pentanoic acid
-
Kocuria rosea
1.4.3.10
160
-
8-amino-1-octanoic acid
-
Kocuria rosea
1.4.3.10
300
-
6-amino-1-hexanoic acid
-
Kocuria rosea
1.4.3.10
440
-
4-amino-1-butanoic acid
-
Kocuria rosea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.10
1,6-diaminohexane + O2 + H2O
1% of the activity with putrescine
395447
Kocuria rosea
?
-
-
-
?
1.4.3.10
cadaverine + O2 + H2O
7% of the activity with putrescine
395447
Kocuria rosea
5-aminopentanal + NH3 + H2O2
-
-
-
?
1.4.3.10
putrescine + O2 + H2O
-
395447
Kocuria rosea
4-aminobutanal + NH3 + H2O2
-
-
-
?
1.4.3.10
spermidine + O2 + H2O
10% of the activity with putrescine
395447
Kocuria rosea
4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
-
-
?