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Literature summary extracted from
- Putrescine oxidase from Micrococcus rubens. Purification and properties of the enzyme (1972), J. Biol. Chem., 247, 5527-5534.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.10 | cadaverine | noncompetitive against putrescine | Kocuria rosea |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.10 | 0.038 | - |
putrescine | - |
Kocuria rosea | |
| 1.4.3.10 | 0.124 | - |
O2 | - |
Kocuria rosea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.10 | 46000 | - |
2 * 46000, SDS-PAGE | Kocuria rosea |
| 1.4.3.10 | 83400 | 87000 | calculation from sedimentation data | Kocuria rosea |
| 1.4.3.10 | 90000 | - |
gel filtration | Kocuria rosea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.3.10 | Kocuria rosea | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.4.3.10 | photoreduction of the enzyme in presence of EDTA | Kocuria rosea |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.3.10 | - |
Kocuria rosea |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.10 | 59.16 | - |
- |
Kocuria rosea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.3.10 | additional information | the enzyme is a weak 2-mercaptoethanol oxidase | Kocuria rosea | ? | - |
? | |
| 1.4.3.10 | putrescine + O2 + H2O | - |
Kocuria rosea | 4-aminobutanal + NH3 + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.3.10 | dimer | 2 * 46000, SDS-PAGE | Kocuria rosea |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.10 | additional information | - |
the enzyme is resistant to heat denaturation as long as it is maintained in a fully reduced state | Kocuria rosea |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.10 | FAD | contains 1 FAD per 82000 Da | Kocuria rosea | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.3.10 | 0.28 | - |
cadaverine | - |
Kocuria rosea | |
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Release 2023.1 (January 2023)
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