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Literature summary extracted from

  • Reddy, R.G.; Yoshimoto, T.; Yamamoto, S.; Funk, C.D.; Marnett, L.J.
    Expression of porcine leukocyte 12-lipoxygenase in a baculovirus/insect cell system and its characterization (1994), Arch. Biochem. Biophys., 312, 219-226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 expression in insect cells using a baculovirus expression vector, expression as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 5,8,11,14-Eicosatetraynoic acid IC50: 0.00006 mM Sus scrofa
1.13.11.31 nordihydroguaiaretic acid IC50: 0.0022 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 0.022
-
 arachidonic acid
-
 Sus scrofa
1.13.11.31 0.03
-
 linoleic acid
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 leukocyte
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.31 0.033
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.13.11.31 -70°C, 20% glycerol, 10 mM 2-mercaptoethanol, 0.3% Triton X-305, enzyme retains complete activity after 1 month Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2 i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid Sus scrofa (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate the 12-(S)-enantiomer is the predominant product ?
1.13.11.31 linoleic acid + O2 19% of the activity with arachidonic acid Sus scrofa 13(S)-hydroperoxy-9Z,11E-octadecadienoic acid
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.31 0.00006
-
 IC50: 0.00006 mM Sus scrofa 5,8,11,14-Eicosatetraynoic acid
1.13.11.31 0.0022
-
 IC50: 0.0022 mM Sus scrofa nordihydroguaiaretic acid