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Literature summary extracted from

  • Richards, K.M.; Marnett, L.J.
    Leukocyte 12-lipoxygenase: Expression, purification, and investigation of the role of methionine residues in turnover-dependent inactivation and 5,8,11,14-eicosatetraynoic acid inhibition (1997), Biochemistry, 36, 6692-6699.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 enzyme is cloned into the expression vectors pSE280, pSE380, and pSE420. PSE380 yields the highest level of 12-lipoxygenase activity, expression in Escherichia coli Top10 cells Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.31 M338L Km-value for arachidonic acid is about 1/2 that of the wild type enzyme Sus scrofa
1.13.11.31 M367V Km-value for arachidonic acid and turnover number are approximately double that of the wild-type enzyme Sus scrofa
1.13.11.31 M562L Km-value for arachidonic acid is 77% of that of the wild type enzyme, turnover number is 85% of that of the wild type Sus scrofa

General Stability

EC Number General Stability Organism
1.13.11.31 15-25% loss of activity upon freezing and thawing Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 5,8,11,14-Eicosatetraynoic acid
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 0.004
-
 arachidonic acid mutant enzyme M338L Sus scrofa
1.13.11.31 0.007
-
 arachidonic acid mutant enzyme M562L Sus scrofa
1.13.11.31 0.009
-
 arachidonic acid wild type enzyme Sus scrofa
1.13.11.31 0.018
-
 arachidonic acid mutant enzyme M367V Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 leukocyte
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.31 12
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.13.11.31 -80°C, stable for at least 4 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2 i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid Sus scrofa (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.31 7.2
-
 arachidonic acid mutant enzyme M561L Sus scrofa
1.13.11.31 8.4
-
 arachidonic acid wild type enzyme and mutant enzyme M338L Sus scrofa
1.13.11.31 18
-
 arachidonic acid mutant enzyme M367V Sus scrofa