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Literature summary extracted from

  • Yokoyama, C.; Shinjo, F.; Yoshimoto, T.; Yamamoto, S.; Oates, J.A.; Brash, A.R.
    Arachidonate 12-lipoxygenase purified from porcine leukocytes by immunoaffinity chromatography and its reactivity with hydroperoxyeicosatetraenoic acids (1986), J. Biol. Chem., 261, 16714-16721.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 arachidonic acid substrate inhibition Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.31 cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.31 72000
-
 x * 72000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 leukocyte
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.31 additional information
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.13.11.31 -70°C, 1 month Sus scrofa
1.13.11.31 4°C, half-life: 24 h, markedly stabilized by 0.02 mM Fe2+ Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
-
 Sus scrofa additional information (8S,15S)-dihydroperoxy-5Z,9E,11Z,13E-eicosatetraenoic acid + (14R,15S)-erythro-dihydroperoxy-5Z,8Z,10E,12E-eicosatetraenoic acid ?
1.13.11.31 5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
-
 Sus scrofa 5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
 ?
1.13.11.31 arachidonate + O2 i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid Sus scrofa (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?
1.13.11.31 gamma-linolenic acid + O2
-
 Sus scrofa 10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
 ?
1.13.11.31 linoleic acid + O2
-
 Sus scrofa 13(S)-hydroperoxy-9Z,11E-octadecadienoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.31 ? x * 72000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.31 4
-
 half-life: 24 h Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 7.5
-
-
 Sus scrofa