BRENDA - Enzyme Database show

Two immunologically and catalytically distinct arachidonate 12-lipoxygenases of bovine platelets and leukocytes

Takahashi, Y.; Ueda, N.; Yamamoto, S.; Arch. Biochem. Biophys. 266, 613-621 (1988)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.31
cytosol
-
Bos taurus
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.31
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.31
partial
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.31
leukocyte
-
Bos taurus
-
1.13.11.31
platelet
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.31
0.0003
-
enzyme from platelet
Bos taurus
1.13.11.31
0.0011
-
enzyme from leukocyte
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.13.11.31
-80°C, stable for at least 1 week
Bos taurus
1.13.11.31
4°C, half-life of leukocyte enzyme is 2 h, half-life of platelet enzyme is 70 h
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.31
11,14,17-eicosatrienoic acid + O2
leukocyte enzyme: 28% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
leukocyte enzyme: 15% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
leukocyte enzyme: 18% of the activity with arachidonic acid, platelet enzyme: 6% of the activity with arachidonic acid
395407
Bos taurus
additional information
-
-
-
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
leukocyte enzyme: 92% of the activity with arachidonic acid, platelet enzyme: 7% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
leukocyte enzyme: 15% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
leukocyte enzyme: 73% of the activity with arachidonic acid, platelet enzyme: less than 94% of the activity with arachidonic acid
395407
Bos taurus
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
leukocyte enzyme: 78% of the activity with arachidonic acid, platelet enzyme: 7% of the activity with arachidonic acid
395407
Bos taurus
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
leukocyte enzyme: 17% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395407
Bos taurus
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395407
Bos taurus
?
1.13.11.31
gamma-linolenic acid + O2
leukocyte enzyme: 32% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
leukocyte enzyme: 11% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.31
7
8
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.31
cytosol
-
Bos taurus
5829
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.31
partial
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.31
leukocyte
-
Bos taurus
-
1.13.11.31
platelet
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.31
0.0003
-
enzyme from platelet
Bos taurus
1.13.11.31
0.0011
-
enzyme from leukocyte
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.13.11.31
-80°C, stable for at least 1 week
Bos taurus
1.13.11.31
4°C, half-life of leukocyte enzyme is 2 h, half-life of platelet enzyme is 70 h
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.31
11,14,17-eicosatrienoic acid + O2
leukocyte enzyme: 28% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
leukocyte enzyme: 15% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
leukocyte enzyme: 18% of the activity with arachidonic acid, platelet enzyme: 6% of the activity with arachidonic acid
395407
Bos taurus
additional information
-
-
-
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
leukocyte enzyme: 92% of the activity with arachidonic acid, platelet enzyme: 7% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
leukocyte enzyme: 15% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
leukocyte enzyme: 73% of the activity with arachidonic acid, platelet enzyme: less than 94% of the activity with arachidonic acid
395407
Bos taurus
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
leukocyte enzyme: 78% of the activity with arachidonic acid, platelet enzyme: 7% of the activity with arachidonic acid
395407
Bos taurus
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
leukocyte enzyme: 17% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
?
-
-
-
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395407
Bos taurus
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395407
Bos taurus
?
1.13.11.31
gamma-linolenic acid + O2
leukocyte enzyme: 32% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
leukocyte enzyme: 11% of the activity with arachidonic acid, platelet enzyme: less than 2% of the activity with arachidonic acid
395407
Bos taurus
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.31
7
8
-
Bos taurus