BRENDA - Enzyme Database

Catalytic properties of human platelet 12-lipoxygenase as compared with the enzymes of other origins

Hada, T.; Ueda, N.; Takahashi, Y.; Yamamoto, S.; Biochim. Biophys. Acta 1083, 89-93 (1991) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.31
0.003
-
5,8,11,14,17-eicosapentaenoic acid
-
Homo sapiens
1.13.11.31
0.0035
-
8,11,14-eicosatrienoic acid
-
Homo sapiens
1.13.11.31
0.008
-
arachidonic acid
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.31
cytosol
-
Homo sapiens
5829
-
1.13.11.31
cytosol
-
Sus scrofa
5829
-
1.13.11.31
cytosol
-
Bos taurus
5829
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.13.11.31
Bos taurus
-
-
-
1.13.11.31
Homo sapiens
-
-
-
1.13.11.31
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.13.11.31
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.31
leukocyte
-
Homo sapiens
-
1.13.11.31
leukocyte
-
Sus scrofa
-
1.13.11.31
leukocyte
-
Bos taurus
-
1.13.11.31
platelet
-
Homo sapiens
-
1.13.11.31
platelet
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.31
0.3
-
-
Homo sapiens
1.13.11.31
0.3
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.13.11.31
11,14,17-eicosatrienoic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
11,14,17-eicosatrienoic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
?
-
-
-
?
1.13.11.31
11,14,17-eicosatrienoic acid + O2
enzyme from leukocyte
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
11,14,17-eicosatrienoic acid + O2
enzyme from leukocyte
395400
Bos taurus
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
enzyme from leukocyte
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
enzyme from leukocyte
395400
Bos taurus
?
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
-
395400
Sus scrofa
additional information
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
-
395400
Bos taurus
additional information
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
enzyme possesses 14,15-leukotriene A4 synthase activity, 8-oxygenase activity and 14-oxygenase activity
395400
Homo sapiens
additional information
14R,15S-dihydroxy-5Z,8Z,10E,12E-eicosatetraenoic acid + 14S,15S-dihydroxy-5Z,8Z,10E,12E-eicosatetraenoic acid + 8S,15S-dihydroxy-5Z,9E,11Z,13E-eicosatetraenoic acid + 8R,15S-dihydroxy-5Z,9E,11Z,13E-eicosatetraenoic acid + 8R,15S-dihydroxy-5Z,9E,11E,13E-eicosatetraenoic acid + 8S,15S-dihydroxy-5Z,9E,11E,13E-eicosatetraenoic acid
395400
Homo sapiens
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
-
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
-
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
-
395400
Bos taurus
?
-
-
-
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
-
395400
Sus scrofa
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
-
-
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
weak activity
395400
Homo sapiens
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
5S,12S-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid and 5S,15S-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid are produced in a ratio of about 8:2
395400
Homo sapiens
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
enzyme from leukocyte, no activity with enzyme from platelet
395400
Bos taurus
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
-
395400
Homo sapiens
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
-
395400
Sus scrofa
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
-
395400
Bos taurus
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
-
395400
Homo sapiens
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
-
395400
Sus scrofa
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
-
395400
Bos taurus
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
?
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
activity with enzyme from leukocyte
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
activity with enzyme from leukocyte
395400
Bos taurus
?
-
-
-
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395400
Homo sapiens
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395400
Homo sapiens
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395400
Sus scrofa
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395400
Sus scrofa
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395400
Bos taurus
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395400
Bos taurus
?
1.13.11.31
gamma-linolenic acid + O2
-
395400
Sus scrofa
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
gamma-linolenic acid + O2
weak activity
395400
Homo sapiens
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
gamma-linolenic acid + O2
enzyme from leukocyte, no activity with enzyme from platelet
395400
Bos taurus
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
enzyme from leukocyte
395400
Sus scrofa
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
enzyme from leukocyte
395400
Bos taurus
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.31
0.003
-
5,8,11,14,17-eicosapentaenoic acid
-
Homo sapiens
1.13.11.31
0.0035
-
8,11,14-eicosatrienoic acid
-
Homo sapiens
1.13.11.31
0.008
-
arachidonic acid
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.31
cytosol
-
Homo sapiens
5829
-
1.13.11.31
cytosol
-
Sus scrofa
5829
-
1.13.11.31
cytosol
-
Bos taurus
5829
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.31
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.31
leukocyte
-
Homo sapiens
-
1.13.11.31
leukocyte
-
Sus scrofa
-
1.13.11.31
leukocyte
-
Bos taurus
-
1.13.11.31
platelet
-
Homo sapiens
-
1.13.11.31
platelet
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.31
0.3
-
-
Homo sapiens
1.13.11.31
0.3
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.13.11.31
11,14,17-eicosatrienoic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
11,14,17-eicosatrienoic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
?
-
-
-
?
1.13.11.31
11,14,17-eicosatrienoic acid + O2
enzyme from leukocyte
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
11,14,17-eicosatrienoic acid + O2
enzyme from leukocyte
395400
Bos taurus
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
enzyme from leukocyte
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
11,14-eicosadienoic acid + O2
enzyme from leukocyte
395400
Bos taurus
?
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
-
395400
Sus scrofa
additional information
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
-
395400
Bos taurus
additional information
-
-
-
?
1.13.11.31
15(S)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid + O2
enzyme possesses 14,15-leukotriene A4 synthase activity, 8-oxygenase activity and 14-oxygenase activity
395400
Homo sapiens
additional information
14R,15S-dihydroxy-5Z,8Z,10E,12E-eicosatetraenoic acid + 14S,15S-dihydroxy-5Z,8Z,10E,12E-eicosatetraenoic acid + 8S,15S-dihydroxy-5Z,9E,11Z,13E-eicosatetraenoic acid + 8R,15S-dihydroxy-5Z,9E,11Z,13E-eicosatetraenoic acid + 8R,15S-dihydroxy-5Z,9E,11E,13E-eicosatetraenoic acid + 8S,15S-dihydroxy-5Z,9E,11E,13E-eicosatetraenoic acid
395400
Homo sapiens
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
-
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
-
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
4,7,10,13,16,19-docosahexaenoic acid + O2
-
395400
Bos taurus
?
-
-
-
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
-
395400
Sus scrofa
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
-
-
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
weak activity
395400
Homo sapiens
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
5S,12S-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid and 5S,15S-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid are produced in a ratio of about 8:2
395400
Homo sapiens
?
1.13.11.31
5(S)-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid + O2
enzyme from leukocyte, no activity with enzyme from platelet
395400
Bos taurus
5,12-(5S,12S)-dihydroxy-6E,8Z,10E,14Z-eicosatetraenoic acid + 5,12-(5S,15S)-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
-
395400
Homo sapiens
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
-
395400
Sus scrofa
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
5,8,11,14,17-eicosapentaenoic acid + O2
-
395400
Bos taurus
12-hydroxy-5,8,10,14,17-eicosapentaenoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
-
395400
Homo sapiens
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
-
395400
Sus scrofa
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
8,11,14-eicosatrienoic acid + O2
-
395400
Bos taurus
12-hydroxyeicosatrienoic acid
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
?
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
?
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
activity with enzyme from leukocyte
395400
Sus scrofa
?
-
-
-
?
1.13.11.31
alpha-linolenic acid + O2
activity with enzyme from leukocyte
395400
Bos taurus
?
-
-
-
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395400
Homo sapiens
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395400
Homo sapiens
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395400
Sus scrofa
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395400
Sus scrofa
?
1.13.11.31
arachidonate + O2
i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid
395400
Bos taurus
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
395400
Bos taurus
?
1.13.11.31
gamma-linolenic acid + O2
-
395400
Sus scrofa
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
gamma-linolenic acid + O2
weak activity
395400
Homo sapiens
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
gamma-linolenic acid + O2
enzyme from leukocyte, no activity with enzyme from platelet
395400
Bos taurus
10-hydroxy-octadeca-6Z,8E,12Z-trienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
no activity with enzyme from platelet
395400
Homo sapiens
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
no activity with enzyme from platelet
395400
Bos taurus
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
enzyme from leukocyte
395400
Sus scrofa
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?
1.13.11.31
linoleic acid + O2
enzyme from leukocyte
395400
Bos taurus
13(S)-hydroxy-9Z,11E-octadecadienoic acid
-
-
-
?