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Literature summary extracted from

  • Rettenmaier, H.; Lingens, F.
    Purification and some properties of two isofunctional juglone hydroxylases from Pseudomonas putida J1 (1985), Biol. Chem. Hoppe-Seyler, 366, 637-646.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.17.3.4 enzyme 1 is not stable in potassium phosphate buffer pH 7.5, 10 vol% methanol, ethanol or propanol stabilizes Pseudomonas putida
1.17.3.4 enzyme 2 is most stable in imidazole/HCl buffer, 10 vol% acetone stabilizes Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.4 3,3'-dithiobis(6-nitrobenzoate) weak inhibition Pseudomonas putida
1.17.3.4 HgCl2 at 0.5 mM complete inhibition of enzyme 1 and enzyme 2 Pseudomonas putida
1.17.3.4 p-chloromercuribenzoate complete inhibition of enzyme 1 and enzyme 2 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.3.4 0.0042
-
 5-hydroxy-1,4-naphthoquinone enzyme 1 Pseudomonas putida
1.17.3.4 0.0185
-
 5-hydroxy-1,4-naphthoquinone enzyme 2 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.3.4 Fe 0.39 atoms per subunit for enzyme 1, 0.04 atoms per subunit for enzyme 2 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.3.4 23500
-
 2 * 23500, enzyme 2, SDS-PAGE Pseudomonas putida
1.17.3.4 25000
-
 2 * 25000, enzyme 1, SDS-PAGE Pseudomonas putida
1.17.3.4 56000
-
 enzyme 2, gel filtration Pseudomonas putida
1.17.3.4 59000
-
 enzyme 1, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.3.4 5-hydroxy-1,4-naphthoquinone + O2 Pseudomonas putida
-
 3,5-dihydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 5-hydroxy-1,4-naphthoquinone + O2 Pseudomonas putida J1
-
 3,5-dihydroxy-1,4-naphthoquinone + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.14 Pseudomonas putida
-
 J1
-
1.13.11.14 Pseudomonas putida J1
-
 J1
-
1.17.3.4 Pseudomonas putida
-
 two isofunctional enzymes named enzyme 1, present in induced and non-induced cells and enzyme 2, present only in juglone-induced cells
-
1.17.3.4 Pseudomonas putida J1
-
 two isofunctional enzymes named enzyme 1, present in induced and non-induced cells and enzyme 2, present only in juglone-induced cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.3.4
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.14 0.095
-
 growth on 2,3-dihydroxybenzoate Pseudomonas putida
1.13.11.14 0.135
-
 growth on glucose Pseudomonas putida
1.17.3.4 242
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.14 2,3-dihydroxybenzoate + O2
-
 Pseudomonas putida CO2 + alpha-hydroxymuconic semialdehyde
-
 ?
1.13.11.14 2,3-dihydroxybenzoate + O2
-
 Pseudomonas putida J1 CO2 + alpha-hydroxymuconic semialdehyde
-
 ?
1.17.3.4 1,4-naphthoquinone + O2
-
 Pseudomonas putida 2-hydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 1,4-naphthoquinone + O2
-
 Pseudomonas putida J1 2-hydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 2-chloro-1,4-naphthoquinone + O2
-
 Pseudomonas putida 2-chloro-3-hydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 2-chloro-1,4-naphthoquinone + O2
-
 Pseudomonas putida J1 2-chloro-3-hydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 5,8-dihydroxy-1,4-naphthoquinone + O2
-
 Pseudomonas putida 2,5,8-trihydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 5,8-dihydroxy-1,4-naphthoquinone + O2
-
 Pseudomonas putida J1 2,5,8-trihydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 5-hydroxy-1,4-naphthoquinone + O2
-
 Pseudomonas putida 3,5-dihydroxy-1,4-naphthoquinone + H2O
-
 ?
1.17.3.4 5-hydroxy-1,4-naphthoquinone + O2
-
 Pseudomonas putida J1 3,5-dihydroxy-1,4-naphthoquinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.3.4 ? 2 * 25000, enzyme 1, SDS-PAGE Pseudomonas putida
1.17.3.4 dimer 2 * 23500, enzyme 2, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.4 7.5 8.5 enzyme 1 and enzyme 2 Pseudomonas putida