EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.1 | Ag+ | 98% inhibition at 0.01 mM | Enterococcus faecalis | |
1.11.1.1 | Cl- | strong inhibitor | Enterococcus faecalis | |
1.11.1.1 | Co2+ | 25% inhibition at 1 mM | Enterococcus faecalis | |
1.11.1.1 | Cu2+ | 85% inhibition at 1 mM | Enterococcus faecalis | |
1.11.1.1 | H2O2 | 43% inhibition at 16.5 mM | Enterococcus faecalis | |
1.11.1.1 | Hg2+ | 21% inhibition at 0.01 mM | Enterococcus faecalis | |
1.11.1.1 | NaN3 | 15% inhibition at 50 mM | Enterococcus faecalis | |
1.11.1.1 | p-chloromercuribenzoate | 20% inhibition at 0.33 mM | Enterococcus faecalis | |
1.11.1.1 | Pb2+ | 49% inhibition at 0.33 mM | Enterococcus faecalis | |
1.11.1.1 | SO42- | strong inhibitor | Enterococcus faecalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.1 | 0.1 | - |
ferricyanide | - |
Enterococcus faecalis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.1 | Fe | small amounts, significance unknown | Enterococcus faecalis | |
1.11.1.1 | additional information | no Mo, Cu, Mn, Zn | Enterococcus faecalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.1 | 120000 | - |
calculation from FAD content | Enterococcus faecalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.1 | NADH + H2O2 | Enterococcus faecalis | - |
NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.1 | Enterococcus faecalis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.1 | partial | Enterococcus faecalis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.1 | additional information | - |
8500-9000 U/ml, calculated as change of 0.01 of optical density | Enterococcus faecalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.1 | ferricyanide + H2O2 | - |
Enterococcus faecalis | ferrocyanide + H2O | - |
? | |
1.11.1.1 | additional information | NADH cannot be replaced by reduced cytochrome c, H2O2 cannot be replaced by Cu2+, Fe3+, SeO32+, NO3-, NO2-, oxidized glutathione, cystine, lipoic acid disulfide, dehydroascorbic acid, cytochrome c, FAD, flavin mononucleotide, riboflavin, 2,6-dichlorophenolindophenol, methylene blue, brilliant cresyl blue | Enterococcus faecalis | ? | - |
? | |
1.11.1.1 | NADH + H2O2 | - |
Enterococcus faecalis | NAD+ + H2O | - |
? | |
1.11.1.1 | NADH + H2O2 | H2O2 can be replaced by 1,4-naphthoquinone which is one third as effective as H2O2 | Enterococcus faecalis | NAD+ + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.1 | 26 | - |
- |
Enterococcus faecalis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.1 | 83.3 | - |
NADH | under standard assay conditions | Enterococcus faecalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.1 | 4.5 | - |
ferricyanide | Enterococcus faecalis |
1.11.1.1 | 5.2 | 5.4 | substrate H2O2 in acetate or phosphate buffer | Enterococcus faecalis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.1 | FAD | FAD-flavoprotein, 1 mol FAD per 1 mol enzyme | Enterococcus faecalis |