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Literature summary extracted from

  • Verhees, C.H.; Tuininga, J.E.; Kengen, S.W.; Stams, A.J.; van der Oost, J.; de Vos, W.M.
    ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea (2001), J. Bacteriol., 183, 7145-7153.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.146 AMP 10 mM, 76% inhibition Methanocaldococcus jannaschii
2.7.1.146 ATP 10 mM, 47% inhibition Methanocaldococcus jannaschii
2.7.1.146 KCl 500 mM, 16% inhibition Methanocaldococcus jannaschii
2.7.1.146 additional information the addition of fructose 2,6-bisphosphate, pyruvate, glucose, phosphoenolpyruvate, or citrate to the assay mixture has no effect on the activity Methanocaldococcus jannaschii
2.7.1.146 NaCl 500 mM, 12% inhibition Methanocaldococcus jannaschii
2.7.1.146 Zn2+ no activity detected in presence of Zn2+ Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.146 0.0096
-
 D-fructose 6-phosphate pH 6.5, 50°C Methanocaldococcus jannaschii
2.7.1.146 0.49
-
 ADP pH 6.5, 50°C Methanocaldococcus jannaschii
2.7.1.146 11.9
-
 acetyl-phosphate pH 6.5, 50°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Ca2+
-
 Methanocaldococcus jannaschii
2.7.1.146 Ca2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii
2.7.1.146 Co2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii
2.7.1.146 Mg2+
-
 Methanocaldococcus jannaschii
2.7.1.146 Mg2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii
2.7.1.146 Mn2+ activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.146 50100
-
 native PAGE Methanocaldococcus jannaschii
2.7.1.146 50100
-
 non-denaturing PAGE Methanocaldococcus jannaschii
2.7.1.146 53362
-
 1 * 53362, calculated from sequence Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate Methanocaldococcus jannaschii key enzyme of the glycolytic pathway AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanocaldococcus jannaschii DSM 2661 key enzyme of the glycolytic pathway AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 D-fructose 6-phosphate + ADP Methanocaldococcus jannaschii ADP can be replaced by acetylphosphate D-fructose 1,6-bisphosphate + AMP
-
 ?
2.7.1.146 D-fructose 6-phosphate + ADP Methanocaldococcus jannaschii ADP can be replaced by acetylphosphate D-fructose 1,6-bisphosphate + AMP
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Methanocaldococcus jannaschii
-
-
-
2.7.1.146 Methanocaldococcus jannaschii Q58999
-
-
2.7.1.146 Methanocaldococcus jannaschii DSM 2661 Q58999
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.146
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.146 8.2
-
-
 Methanocaldococcus jannaschii
2.7.1.146 8.2
-
 pH 6.5, 50°C, direction of phosphorylation Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 acetyl-phosphate + D-fructose 6-phosphate activity is 83% compared to the activity with ADP Methanocaldococcus jannaschii acetate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 acetyl-phosphate + D-fructose 6-phosphate activity is 83% compared to the activity with ADP Methanocaldococcus jannaschii DSM 2661 acetate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate key enzyme of the glycolytic pathway Methanocaldococcus jannaschii AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate Methanocaldococcus jannaschii AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate key enzyme of the glycolytic pathway Methanocaldococcus jannaschii DSM 2661 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate Methanocaldococcus jannaschii DSM 2661 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 D-fructose 6-phosphate + ADP ADP can be replaced by acetylphosphate Methanocaldococcus jannaschii D-fructose 1,6-bisphosphate + AMP
-
 ?
2.7.1.146 D-fructose 6-phosphate + ADP ADP can be replaced by acetylphosphate Methanocaldococcus jannaschii D-fructose 1,6-bisphosphate + AMP
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.146 monomer 1 * 53362, calculated from sequence Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.146 ADP-dependent PFK
-
 Methanocaldococcus jannaschii
2.7.1.146 ADP-Pfk
-
 Methanocaldococcus jannaschii
2.7.1.146 MJ1604 MJ1604 Methanocaldococcus jannaschii
2.7.1.146 pfkC
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.146 50
-
 assay at Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.146 6.5
-
 assay at Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.7.1.146 physiological function key enzyme of the modified Embden-Meyerhof pathway of heterotrophic and chemolithoautotrophic archaea Methanocaldococcus jannaschii