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Literature summary extracted from

  • Shapleigh, J.P.; Davies, K.J.P.; Payne, W.J.
    Detergent inhibition of nitric-oxide reductase activity (1987), Biochim. Biophys. Acta, 911, 334-340.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.5 Triton X-100 7.5% Triton X-100 results in complete inactivation after 6 h Achromobacter cycloclastes
1.7.2.5 Triton X-100 5% Triton X-100 results in complete inactivation after 4-5 h Paracoccus denitrificans
1.7.2.5 Triton X-100 5% Triton X-100 results in complete inactivation after 4-5 h Pseudomonas aeruginosa
1.7.2.5 Triton X-100 5% Triton X-100 results in complete inactivation after 4-5 h Stutzerimonas stutzeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 membrane
-
 Paracoccus denitrificans 16020
-
1.7.2.5 membrane
-
 Achromobacter cycloclastes 16020
-
1.7.2.5 membrane
-
 Pseudomonas aeruginosa 16020
-
1.7.2.5 membrane
-
 Stutzerimonas stutzeri 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor Paracoccus denitrificans
-
 nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor Pseudomonas aeruginosa
-
 nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor Achromobacter cycloclastes
-
 nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor Stutzerimonas stutzeri
-
 nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor Pseudomonas aeruginosa PAO 1
-
 nitrous oxide + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Achromobacter cycloclastes
-
 strain ATCC 21921
-
1.7.2.5 Achromobacter cycloclastes
-
-
-
1.7.2.5 Paracoccus denitrificans
-
 strain ATCC 19367
-
1.7.2.5 Paracoccus denitrificans
-
-
-
1.7.2.5 Pseudomonas aeruginosa
-
-
-
1.7.2.5 Pseudomonas aeruginosa PAO 1
-
-
-
1.7.2.5 Stutzerimonas stutzeri
-
 strain ATCC 14405
-
1.7.2.5 Stutzerimonas stutzeri
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.5 0.0021
-
 with ascorbate and 2,3,5,6-tetramethyl-1,4-benzoquinone as electron donors Stutzerimonas stutzeri
1.7.2.5 0.0028
-
 with ascorbate and 2,3,5,6-tetramethyl-1,4-benzoquinone as electron donors Achromobacter cycloclastes
1.7.2.5 0.0033
-
 with ascorbate and 2,3,5,6-tetramethyl-1,4-benzoquinone as electron donors Paracoccus denitrificans
1.7.2.5 0.004
-
 with ascorbate and 2,3,5,6-tetramethyl-1,4-benzoquinone as electron donors Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Pseudomonas aeruginosa nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Achromobacter cycloclastes nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Stutzerimonas stutzeri nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Pseudomonas aeruginosa nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Achromobacter cycloclastes nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Stutzerimonas stutzeri nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Pseudomonas aeruginosa PAO 1 nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Pseudomonas aeruginosa PAO 1 nitrous oxide + acceptor + H2O
-
 ?