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Literature summary extracted from

  • Carr, G.J.; Page, M.D.; Ferguson, S.J.
    The energy-conserving nitric-oxide-reductase system in Paracoccus denitrificans. Distinction from the nitrite reductase that catalyses synthesis of nitric oxide and evidence from trapping experiments for nitric oxide as a free intermediate during denitrification (1989), Eur. J. Biochem., 179, 683-692.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.2.5 after one cycle of freezing to -20°C and thawing stable, after more cycles activity is lost Paracoccus denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.5 Antimycin 100% inhibition at 0.005 mg per mg vesicle protein, no inhibition with isoascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Paracoccus denitrificans
1.7.2.5 Myxothiazol 100% inhibition at 200 ng per mg vesicle protein, no inhibition with isoascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 membrane
-
 Paracoccus denitrificans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 Fe
-
 Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor Paracoccus denitrificans
-
 nitrous oxide + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Paracoccus denitrificans
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.5 0.58
-
-
 Paracoccus denitrificans

Storage Stability

EC Number Storage Stability Organism
1.7.2.5 -20°C, stable for 2 months Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor NADH, succinate or isoascorbate plus 2,3,5,6-tetramethyl-1,4-benzendiamine as electron donors Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 heme
-
 Paracoccus denitrificans
1.7.2.5 NADH
-
 Paracoccus denitrificans