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Literature summary extracted from

  • Dermastia, M.; Turk, T.; Hollocher, T.C.
    Nitric oxide reductase. Purification from Paracoccus denitrificans with use of a single column and some characteristics [published erratum appears in J Biol Chem 1991 Aug 25;266(24):16256] (1991), J. Biol. Chem., 266, 10899-10905.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.2.5 0.8% octyl glucoside is needed for solubilzation Paracoccus denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.5 NO reversible inhibition Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 Fe
-
 Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor Paracoccus denitrificans
-
 nitrous oxide + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Paracoccus denitrificans
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.5 10
-
 at pH 6.5 Paracoccus denitrificans
1.7.2.5 22
-
 at pH 5.0 Paracoccus denitrificans

Storage Stability

EC Number Storage Stability Organism
1.7.2.5 -20°C, stable Paracoccus denitrificans
1.7.2.5 4°C, 2 d, 50% activity, the inclusion of 0.01% 2-phenylethanol, 1 mM benzamidine and 100 nM pepstatin stabilizes Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate/phenazine methosulfate, N,N,N',N'-tetramethyl-1,4-benzendiamine/ascorbate, 2,3,5,6-tetramethyl-1,4-benzendiamine/ascorbate and NADH as electron donors Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.5 5
-
-
 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 heme
-
 Paracoccus denitrificans
1.7.2.5 NADH
-
 Paracoccus denitrificans