BRENDA - Enzyme Database show

Nitric oxide reductase from Pseudomonas stutzeri, a novel cytochrome bc complex. Phospholipid requirement, electron paramagnetic resonance and redox properties

Kastrau, D.H.W.; Heiss, B.; Kroneck, P.M.H.; Zumft, W.G.; Eur. J. Biochem. 222, 293-303 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.7.2.5
cardiolipin
-
Pseudomonas stutzeri
1.7.2.5
phosphatidylglycerol
-
Pseudomonas stutzeri
General Stability
EC Number
General Stability
Organism
1.7.2.5
a phospholipid is required for high catalytic activity
Pseudomonas stutzeri
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.2.5
elaidic acid
-
Pseudomonas stutzeri
1.7.2.5
linoleic acid
-
Pseudomonas stutzeri
1.7.2.5
linolenic acid
-
Pseudomonas stutzeri
1.7.2.5
oleic acid
-
Pseudomonas stutzeri
1.7.2.5
palmitoleic acid
-
Pseudomonas stutzeri
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.7.2.5
Fe
-
Pseudomonas stutzeri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.2.5
17000
-
1 * 17000 + 1 * 38000, SDS-PAGE
Pseudomonas stutzeri
1.7.2.5
38000
-
1 * 17000 + 1 * 38000, SDS-PAGE
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.7.2.5
2 nitric oxide + reduced acceptor
Pseudomonas stutzeri
-
nitrous oxide + acceptor + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.7.2.5
Pseudomonas stutzeri
-
ZoBell strain, ATCC 14405
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.7.2.5
lipoprotein
contains cardiolipin, phosphatidylglycerol and phosphatidylethanolamine
Pseudomonas stutzeri
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.7.2.5
60
-
ascorbate and phenazine methosulfate as electron donors
Pseudomonas stutzeri
Storage Stability
EC Number
Storage Stability
Organism
1.7.2.5
17░C, 0.1% laurylpropanediol-3-phosphorylcholine ether or 0.1% dodecylmaltoside, 8 days, 100% activity
Pseudomonas stutzeri
1.7.2.5
17░C, Triton X-100, 2 days, 100% activity
Pseudomonas stutzeri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.2.5
2 nitric oxide + reduced acceptor
-
395132
Pseudomonas stutzeri
nitrous oxide + acceptor + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.7.2.5
dimer
1 * 17000 + 1 * 38000, SDS-PAGE
Pseudomonas stutzeri
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.2.5
4.8
-
-
Pseudomonas stutzeri
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.7.2.5
4.8
6
at pH 6.0 half of the activity of pH 4.8
Pseudomonas stutzeri
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.7.2.5
6.5
-
inactivation after 1 h at room temperature
Pseudomonas stutzeri
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.2.5
heme
-
Pseudomonas stutzeri
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.7.2.5
0.0065
-
oleic acid
-
Pseudomonas stutzeri
1.7.2.5
0.0098
-
palmitoleic acid
-
Pseudomonas stutzeri
1.7.2.5
0.0125
-
linoleic acid
-
Pseudomonas stutzeri
1.7.2.5
0.014
-
linolenic acid
-
Pseudomonas stutzeri
1.7.2.5
0.0235
-
elaidic acid
-
Pseudomonas stutzeri
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.7.2.5
cardiolipin
-
Pseudomonas stutzeri
1.7.2.5
phosphatidylglycerol
-
Pseudomonas stutzeri
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.2.5
heme
-
Pseudomonas stutzeri
General Stability (protein specific)
EC Number
General Stability
Organism
1.7.2.5
a phospholipid is required for high catalytic activity
Pseudomonas stutzeri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.2.5
elaidic acid
-
Pseudomonas stutzeri
1.7.2.5
linoleic acid
-
Pseudomonas stutzeri
1.7.2.5
linolenic acid
-
Pseudomonas stutzeri
1.7.2.5
oleic acid
-
Pseudomonas stutzeri
1.7.2.5
palmitoleic acid
-
Pseudomonas stutzeri
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.7.2.5
0.0065
-
oleic acid
-
Pseudomonas stutzeri
1.7.2.5
0.0098
-
palmitoleic acid
-
Pseudomonas stutzeri
1.7.2.5
0.0125
-
linoleic acid
-
Pseudomonas stutzeri
1.7.2.5
0.014
-
linolenic acid
-
Pseudomonas stutzeri
1.7.2.5
0.0235
-
elaidic acid
-
Pseudomonas stutzeri
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.7.2.5
Fe
-
Pseudomonas stutzeri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.2.5
17000
-
1 * 17000 + 1 * 38000, SDS-PAGE
Pseudomonas stutzeri
1.7.2.5
38000
-
1 * 17000 + 1 * 38000, SDS-PAGE
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.7.2.5
2 nitric oxide + reduced acceptor
Pseudomonas stutzeri
-
nitrous oxide + acceptor + H2O
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.7.2.5
lipoprotein
contains cardiolipin, phosphatidylglycerol and phosphatidylethanolamine
Pseudomonas stutzeri
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.7.2.5
60
-
ascorbate and phenazine methosulfate as electron donors
Pseudomonas stutzeri
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.7.2.5
17░C, 0.1% laurylpropanediol-3-phosphorylcholine ether or 0.1% dodecylmaltoside, 8 days, 100% activity
Pseudomonas stutzeri
1.7.2.5
17░C, Triton X-100, 2 days, 100% activity
Pseudomonas stutzeri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.2.5
2 nitric oxide + reduced acceptor
-
395132
Pseudomonas stutzeri
nitrous oxide + acceptor + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.7.2.5
dimer
1 * 17000 + 1 * 38000, SDS-PAGE
Pseudomonas stutzeri
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.2.5
4.8
-
-
Pseudomonas stutzeri
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.7.2.5
4.8
6
at pH 6.0 half of the activity of pH 4.8
Pseudomonas stutzeri
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.7.2.5
6.5
-
inactivation after 1 h at room temperature
Pseudomonas stutzeri