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Literature summary extracted from

  • Girsch, P.; de Vries, S.
    Purification and initial kinetic and spectroscopic characterization of NO reductase from Paracoccus denitrificans (1997), Biochim. Biophys. Acta, 1318, 202-216.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.5 NO substrate inhibition Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 membrane
-
Paracoccus denitrificans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 Fe 2 heme b, 1 heme c and 1 non-heme Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.5 17000
-
1 * 37000 + 1 * 17000, SDS-PAGE Paracoccus denitrificans
1.7.2.5 37000
-
1 * 37000 + 1 * 17000, SDS-PAGE Paracoccus denitrificans
1.7.2.5 69500
-
DNA sequence Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor Paracoccus denitrificans
-
nitrous oxide + acceptor + H2O
-
?
1.7.2.5 2 nitric oxide + reduced acceptor Paracoccus denitrificans Pd 1222
-
nitrous oxide + acceptor + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Paracoccus denitrificans
-
-
-
1.7.2.5 Paracoccus denitrificans Pd 1222
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.5 using detergent lauryl maltoside Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.5 25.8
-
with ascorbate, phenazine methosulfate and cytochrome c as electron donors Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor
-
Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
?
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate, phenazine methosulfate and horse heart cytochrome c as the electron donor/mediator system Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
?
1.7.2.5 2 nitric oxide + reduced acceptor cytochrome c as mediator Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
?
1.7.2.5 2 nitric oxide + reduced acceptor
-
Paracoccus denitrificans Pd 1222 nitrous oxide + acceptor + H2O
-
?
1.7.2.5 2 nitric oxide + reduced acceptor ascorbate, phenazine methosulfate and horse heart cytochrome c as the electron donor/mediator system Paracoccus denitrificans Pd 1222 nitrous oxide + acceptor + H2O
-
?
1.7.2.5 2 nitric oxide + reduced acceptor cytochrome c as mediator Paracoccus denitrificans Pd 1222 nitrous oxide + acceptor + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.7.2.5 dimer cytochrome bc complex, subunits named NorB and NorC Paracoccus denitrificans
1.7.2.5 dimer 1 * 37000 + 1 * 17000, SDS-PAGE Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 heme
-
Paracoccus denitrificans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.2.5 0.0135
-
NO
-
Paracoccus denitrificans