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Literature summary extracted from

  • Sprott, G.D.; Shaw, K.M.; Beveridge, T.J.
    Properties of the particulate enzyme F420-reducing hydrogenase isolated from Methanospirillum hungatei (1987), Can. J. Microbiol., 33, 896-904.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.12.98.1 freezing and thawing changes properties of enzyme, different behavior in anion exchange chromatography Methanospirillum hungatei
1.12.98.1 unstable in prereduced buffer, dramatic decline of activity after 2 h Methanospirillum hungatei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 Ca2+ activating Methanospirillum hungatei
1.12.98.1 Iron
-
 Methanothermobacter thermautotrophicus
1.12.98.1 Iron non-heme iron Methanospirillum hungatei
1.12.98.1 Mg2+ activating Methanospirillum hungatei
1.12.98.1 Nickel
-
 Methanothermobacter thermautotrophicus
1.12.98.1 Nickel
-
 Methanospirillum hungatei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 30700
-
 alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy Methanospirillum hungatei
1.12.98.1 50700
-
 alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy Methanospirillum hungatei
1.12.98.1 720000
-
-
 Methanospirillum hungatei
1.12.98.1 960000
-
 gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanothermobacter thermautotrophicus enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 Methanospirillum hungatei enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanospirillum hungatei
-
-
-
1.12.98.1 Methanothermobacter thermautotrophicus
-
 strain delta H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1
-
 Methanothermobacter thermautotrophicus
1.12.98.1
-
 Methanospirillum hungatei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 0.847
-
 purified enzyme, F420-linked activity Methanospirillum hungatei
1.12.98.1 11.1
-
 purified enzyme, benzyl viologen-linked activity Methanospirillum hungatei

Storage Stability

EC Number Storage Stability Organism
1.12.98.1 liquid nitrogen, in presence of 50% sucrose and 10% glycerol, stable Methanospirillum hungatei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + benzyl viologen
-
 Methanospirillum hungatei ?
-
 ?
1.12.98.1 H2 + coenzyme F420
-
 Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420
-
 Methanospirillum hungatei reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanospirillum hungatei reduced coenzyme F420
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma 45000 + 31000 + 28000, SDS-PAGE, exact subunit composition not known Methanothermobacter thermautotrophicus
1.12.98.1 multimer alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy Methanospirillum hungatei

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.1 additional information no requirement for added FAD Methanospirillum hungatei