Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jin, S.L.C.; Blanchard, D.K.; Chen, J.S.
    Two hydrogenases with distinct electron-carrier specificity and subunit composition in Methanobacterium formicicum (1983), Biochim. Biophys. Acta, 748, 8-20.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 150000
-
 gel filtration Methanobacterium formicicum
1.12.98.1 380000
-
 gel filtration Methanobacterium formicicum
1.12.98.1 600000
-
 gel filtration Methanobacterium formicicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanobacterium bryantii enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 Methanobacterium formicicum enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 Methanobacterium formicicum MF enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanobacterium bryantii
-
-
-
1.12.98.1 Methanobacterium formicicum
-
-
-
1.12.98.1 Methanobacterium formicicum MF
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.1 more stable under aerobic conditions, reversible loss of activity with oxygen can be restored with dithionite Methanobacterium formicicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 0.0014
-
 methyl viologen-linked activity Methanobacterium bryantii
1.12.98.1 0.0026
-
 methyl viologen-linked activity Methanobacterium formicicum
1.12.98.1 0.032
-
 F420-linked activity Methanobacterium formicicum
1.12.98.1 0.155
-
 F420-linked activity Methanobacterium bryantii

Storage Stability

EC Number Storage Stability Organism
1.12.98.1 4°C, 4 weeks, 25% loss of activity towards F420, 97% loss of activity towards methyl viologen Methanobacterium formicicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F420
-
 Methanobacterium bryantii reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420
-
 Methanobacterium formicicum reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanobacterium bryantii reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanobacterium formicicum reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420
-
 Methanobacterium formicicum MF reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanobacterium formicicum MF reduced coenzyme F420
-
 ?
1.12.98.1 H2 + methyl viologen
-
 Methanobacterium bryantii ?
-
 ?
1.12.98.1 H2 + methyl viologen
-
 Methanobacterium formicicum ?
-
 ?
1.12.98.1 H2 + methyl viologen
-
 Methanobacterium formicicum MF ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma 42600 + 23500 + 35000, SDS-PAGE, exact subunit composition not known Methanobacterium formicicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.98.1
-
 5 reactivated enzyme stable Methanobacterium formicicum
1.12.98.1 25 30 reactivated enzyme unstable Methanobacterium formicicum