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Literature summary extracted from

  • Yamazaki, S.
    A selenium-containing hydrogenase from Methanococcus vannielii. Identification of the selenium moiety as a selenocysteine residue (1982), J. Biol. Chem., 257, 7926-7929.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 selenium as selenocysteine, only present in 42000 D subunit Methanococcus vannielii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 340000
-
 gel filtration, enzyme tends to aggregate, aggregated form MW 1300000 Methanococcus vannielii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanococcus vannielii enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanococcus vannielii
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.1 extremely oxygen sensitive, can be reactivated by incubation with molecular hydrogen and dithiothreitol Methanococcus vannielii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1
-
 Methanococcus vannielii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F420
-
 Methanococcus vannielii reduced coenzyme F420
-
 ?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanococcus vannielii reduced coenzyme F420
-
 ?
1.12.98.1 additional information reduces tetrazolium dyes with molecular oxygen Methanococcus vannielii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 multimer alpha,beta,gamma 56000 + 42000 + 35000, SDS-PAGE, three subunit species found but exact subunit composition not known Methanococcus vannielii