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Literature summary extracted from

  • Yamazaki, T.; Oyanagi, H.; Fujiwara, T.; Fukumori, Y.
    Nitrite reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum. A novel cytochrome cd1 with Fe(II):nitrite oxidoreductase activity (1995), Eur. J. Biochem., 233, 665-671.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.1 CN- 0.047 mM, 50% inhibition of nitrite reduction Magnetospirillum magnetotacticum
1.7.2.1 CN-
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 0.00147
-
 NO2-
-
 Magnetospirillum magnetotacticum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.1 periplasm
-
 Pseudomonas aeruginosa
-
-
1.7.2.1 periplasm 75% of enzyme activity found in the soluble fraction Magnetospirillum magnetotacticum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 54000
-
 2 * 54000, SDS-PAGE Magnetospirillum magnetotacticum
1.7.2.1 133000
-
 gel filtration Magnetospirillum magnetotacticum

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Magnetospirillum magnetotacticum
-
 formerly Aquaspirillum magnetotacticum
-
1.7.2.1 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 DEAE-Toyopearl, CM-cellulose, Sephacryl S-200 Magnetospirillum magnetotacticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 3.32
-
 reduction of N,N,N',N'-tetramethyl-4-phenyldiamine Magnetospirillum magnetotacticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 ferrocytochrome c-551 + O2
-
 Pseudomonas aeruginosa H2O + ferricytochrome c-551
-
 ?
1.7.2.1 ferrocytochrome c-551 + O2
-
 Magnetospirillum magnetotacticum H2O + ferricytochrome c-551
-
 ?
1.7.2.1 reduced tetramethyl-4-phenylenediamine + NO2 no reaction with horse ferrocytochrome c, Neurospora europaea ferrocytochrome c-552, Magnetospirillum magnetotacticum ferrocytochrome c-550 and Pseudomonas aeruginosa cytochrome c-551 Magnetospirillum magnetotacticum oxidized tetrametyl-4-phenylenediamine + NO
-
 ?
1.7.2.1 reduced tetramethyl-4-phenylenediamine + O2
-
 Magnetospirillum magnetotacticum oxidized tetrametyl-4-phenylenediamine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.1 dimer
-
 Pseudomonas aeruginosa
1.7.2.1 dimer 2 * 54000, SDS-PAGE Magnetospirillum magnetotacticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.1 80
-
 NO2-
-
 Pseudomonas aeruginosa
1.7.2.1 80
-
 NO2- electron donor: reduced tetramethyl-4-phenylenediamine Magnetospirillum magnetotacticum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.1 heme c
-
 Pseudomonas aeruginosa
1.7.2.1 heme c
-
 Magnetospirillum magnetotacticum
1.7.2.1 Heme d1
-
 Pseudomonas aeruginosa
1.7.2.1 Heme d1
-
 Magnetospirillum magnetotacticum