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Literature summary extracted from

  • Chang, S.H.; Wilken, D.R.
    Participation of the unsymmetrical disulfide of coenzyme A and glutathione in an enzymatic sulfhydryl-disulfide interchange (1966), J. Biol. Chem., 241, 4251-4260.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.4.3 inactivation during storage is largely reversed by glutathione Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.3 0.045
-
 CoA-glutathione
-
 Bos taurus
1.8.4.3 0.33
-
 GSH
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.3 120000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.3 CoA-glutathione + GSH Rattus norvegicus
-
 CoA + GSSG
-
 r
1.8.4.3 CoA-glutathione + GSH Bos taurus
-
 CoA + GSSG
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.3 Bos taurus
-
-
-
1.8.4.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.3
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.3 brain
-
 Rattus norvegicus
-
1.8.4.3 heart low activity Rattus norvegicus
-
1.8.4.3 kidney
-
 Rattus norvegicus
-
1.8.4.3 kidney
-
 Bos taurus
-
1.8.4.3 liver
-
 Rattus norvegicus
-
1.8.4.3 lung
-
 Rattus norvegicus
-
1.8.4.3 muscle
-
 Rattus norvegicus
-
1.8.4.3 pancreas
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.3 0.513
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.8.4.3 -12°C, 6 weeks, stable Bos taurus
1.8.4.3 -12°C, 7 months, 70% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.3 CoA-glutathione + GSH
-
 Rattus norvegicus CoA + GSSG
-
 r
1.8.4.3 CoA-glutathione + GSH
-
 Bos taurus CoA + GSSG
-
 r
1.8.4.3 cysteine-glutathione + GSH
-
 Rattus norvegicus cysteine + GSSG
-
 ?
1.8.4.3 cysteine-glutathione + GSH
-
 Bos taurus cysteine + GSSG
-
 ?
1.8.4.3 additional information possible functions: 1. salvage of the coenzyme form of pantothenic acid which might be lost to the cell as CoA-glutathione, 2. maintenance of the free CoA-SH at cellular level Rattus norvegicus ?
-
 ?
1.8.4.3 additional information possible functions: 1. salvage of the coenzyme form of pantothenic acid which might be lost to the cell as CoA-glutathione, 2. maintenance of the free CoA-SH at cellular level Bos taurus ?
-
 ?
1.8.4.3 additional information catalyzes GSH-GSSG exchange reaction Rattus norvegicus ?
-
 ?
1.8.4.3 additional information catalyzes GSH-GSSG exchange reaction Bos taurus ?
-
 ?
1.8.4.3 pantetheine-glutathione + GSH
-
 Bos taurus pantetheine + GSSG
-
 ?
1.8.4.3 thiolethanolamine-glutathione + GSH
-
 Bos taurus thioethanolamine + GSSG
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.3 25
-
 assay at Rattus norvegicus
1.8.4.3 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.3 8.2
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.4.3 7 9 pH 7: about 30% of activity maximum, pH 9: about 75% of activity maximum Bos taurus