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Literature summary extracted from
- Purification and characterization of catechol 1,2-dioxygenase from Rhodococcus rhodochrous NCIMB 13259 and cloning and sequencing of its catA gene (1998), Biochem. J., 333, 741-747.
No PubMed abstract available
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.13.11.1 | sitting-drop vapour-diffusion technique, X-ray characterization, space group C2, unit-cell dimensions: a = 111.9 a, b = 78.1 A, c = 134.6 A, beta = 100° | Rhodococcus rhodochrous |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.1 | 0.0011 | - |
catechol | - |
Rhodococcus rhodochrous |  |
| 1.13.11.1 | 0.0012 | - |
4-methylcatechol | - |
Rhodococcus rhodochrous | |
| 1.13.11.1 | 0.0017 | - |
3-methylcatechol | - |
Rhodococcus rhodochrous | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.1 | Iron | 0.60-0.65 iron per subunit | Rhodococcus rhodochrous | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 31558 | - |
4 * 31558, electrospray mass spectrometry | Rhodococcus rhodochrous |
| 1.13.11.1 | 120000 | - |
gel filtration | Rhodococcus rhodochrous |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.1 | Rhodococcus rhodochrous | - |
NCIMB 13259 | - |
| 1.13.11.1 | Rhodococcus rhodochrous NCIMB 13259 | - |
NCIMB 13259 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.1 | - |
Rhodococcus rhodochrous |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 14.3 | - |
- |
Rhodococcus rhodochrous |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.1 | 3-methylcatechol + O2 | - |
Rhodococcus rhodochrous | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 1.13.11.1 | 3-methylcatechol + O2 | - |
Rhodococcus rhodochrous NCIMB 13259 | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 1.13.11.1 | 4-methylcatechol + O2 | - |
Rhodococcus rhodochrous | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| 1.13.11.1 | 4-methylcatechol + O2 | - |
Rhodococcus rhodochrous NCIMB 13259 | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| 1.13.11.1 | catechol + O2 | - |
Rhodococcus rhodochrous | cis,cis-muconate | - |
? | |
| 1.13.11.1 | catechol + O2 | - |
Rhodococcus rhodochrous NCIMB 13259 | cis,cis-muconate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.1 | tetramer | 4 * 31558, electrospray mass spectrometry | Rhodococcus rhodochrous |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.1 | 6.3 | - |
4-methylcatechol | - |
Rhodococcus rhodochrous | |
| 1.13.11.1 | 9.5 | - |
3-methylcatechol | - |
Rhodococcus rhodochrous | |
| 1.13.11.1 | 11.6 | - |
catechol | - |
Rhodococcus rhodochrous | |
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