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Literature summary extracted from

  • Briganti, F.; Pessione, E.; Giunta, C.; Scozzafava, A.
    Purification, biochemical properties and substrate specificity of a catechol 1,2-dioxygenase from a phenol degrading Acinetobacter radioresistens (1997), FEBS Lett., 416, 61-64.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0032
-
 catechol
-
 Acinetobacter radioresistens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 0.96 mol per mol of protein Acinetobacter radioresistens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 78000 79000 PAGE, gel filtration Acinetobacter radioresistens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Acinetobacter radioresistens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Acinetobacter radioresistens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 24.5
-
-
 Acinetobacter radioresistens

Storage Stability

EC Number Storage Stability Organism
1.13.11.1 4°C, 100% activity after 1 month Acinetobacter radioresistens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2
-
 Acinetobacter radioresistens (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Acinetobacter radioresistens (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Acinetobacter radioresistens cis,cis-muconate
-
 ?
1.13.11.1 additional information both intradiol and extradiol cleavage of 3-methylcatechol Acinetobacter radioresistens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 homodimer 2 * 38700-39000, at low ionic strength, upon increasing the ionic strength the enzyme monomerizes, SDS-PAGE Acinetobacter radioresistens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 37 47
-
 Acinetobacter radioresistens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 15.8
-
 catechol
-
 Acinetobacter radioresistens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 6 8.5 the activity rapidly decreases outside this range Acinetobacter radioresistens