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Literature summary extracted from

  • Tsukada, K.
    D-Amino acid dehydrogenase (Pseudomonas fluorescens) (1971), Methods Enzymol., 17B, 623-624.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.4.99.6 very unstable Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.99.6 2,6-dichlorophenolindophenol inhibition of the dichlorophenolindophenol-specific enzyme at high concentrations Pseudomonas fluorescens
1.4.99.6 D-Amino acids various D-amino acids competitively inhibit D-kynurenine deamination Pseudomonas fluorescens
1.4.99.6 p-chloromercuribenzoic acid 90% inhibition of the methylene blue specific, 70% inhibition of the dichlorophenolindophenol specific enzyme at 0.5 mM Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.99.6 0.008
-
methylene blue specific acceptor, cosubstrate: D-kynurenine Pseudomonas fluorescens
1.4.99.6 0.07
-
2,6-dichlorophenolindophenol specific acceptor, cosubstrate: D-kynurenine Pseudomonas fluorescens
1.4.99.6 0.45
-
D-kynurenine both methylene blue and 2,6-dichlorophenolindophenol specific enzymes Pseudomonas fluorescens
1.4.99.6 0.47
-
D-tryptophan both methylene blue and 2,6-dichlorophenolindophenol specific enzymes Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.6 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.99.6 near homogeneity, two enzymes with different acceptor specificities, fractionation and chromatography techniques Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.99.6 cell culture one methylene blue specific, one 2,6-dichlorophenolindophenol specific enzyme, the methylene blue specific enzyme is only detectable in D-tryptophan grown cells Pseudomonas fluorescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.99.6 0.113
-
methylene blue specific enzyme Pseudomonas fluorescens
1.4.99.6 0.213
-
2,6-dichlorophenolindophenol specific enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.6 D-alanine + H2O + 2,6-dichlorophenolindophenol low activity Pseudomonas fluorescens pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-alanine + H2O + methylene blue low activity Pseudomonas fluorescens pyruvate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-asparagine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-asparagine + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxosuccinamic acid + NH3 + reduced methylene blue
-
?
1.4.99.6 D-cysteine + H2O + methylene blue
-
Pseudomonas fluorescens 3-mercapto-2-oxopropanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-cystine + H2O + methylene blue
-
Pseudomonas fluorescens ? + NH3 + reduced methylene blue
-
?
1.4.99.6 D-histidine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-histidine + H2O + methylene blue
-
Pseudomonas fluorescens 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-isoleucine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-isoleucine + H2O + methylene blue
-
Pseudomonas fluorescens 3-methyl-2-oxopentanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-kynurenine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-kynurenine + H2O + methylene blue
-
Pseudomonas fluorescens 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-leucine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-leucine + H2O + methylene blue
-
Pseudomonas fluorescens 4-methyl-2-oxopentanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-methionine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-norleucine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 2-oxohexanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-norleucine + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxohexanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-norvaline + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxopentanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-phenylalanine + H2O + methylene blue
-
Pseudomonas fluorescens phenylpyruvate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-serine + H2O + 2,6-dichlorophenolindophenol low activity Pseudomonas fluorescens 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-serine + H2O + methylene blue low activity Pseudomonas fluorescens 3-hydroxy-2-oxopropanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-threonine + H2O + 2,6-dichlorophenolindophenol low activity Pseudomonas fluorescens 3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-threonine + H2O + methylene blue low activity Pseudomonas fluorescens 3-hydroxy-2-oxobutanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-tryptophan + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-tryptophan + H2O + methylene blue
-
Pseudomonas fluorescens 3-indole-2-oxopropanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-tyrosine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-tyrosine + H2O + methylene blue
-
Pseudomonas fluorescens 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced methylene blue
-
?
1.4.99.6 D-valine + H2O + 2,6-dichlorophenolindophenol
-
Pseudomonas fluorescens 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-valine + H2O + methylene blue
-
Pseudomonas fluorescens 2-oxoisopentanoate + NH3 + reduced methylene blue
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.99.6 7 8 both methylene blue and 2,6-dichlorophenolindophenol specific enzymes Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.99.6 FAD
-
Pseudomonas fluorescens