EC Number | General Stability | Organism |
---|---|---|
1.4.99.6 | very unstable | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.99.6 | 2,6-dichlorophenolindophenol | inhibition of the dichlorophenolindophenol-specific enzyme at high concentrations | Pseudomonas fluorescens | |
1.4.99.6 | D-Amino acids | various D-amino acids competitively inhibit D-kynurenine deamination | Pseudomonas fluorescens | |
1.4.99.6 | p-chloromercuribenzoic acid | 90% inhibition of the methylene blue specific, 70% inhibition of the dichlorophenolindophenol specific enzyme at 0.5 mM | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.6 | 0.008 | - |
methylene blue | specific acceptor, cosubstrate: D-kynurenine | Pseudomonas fluorescens | |
1.4.99.6 | 0.07 | - |
2,6-dichlorophenolindophenol | specific acceptor, cosubstrate: D-kynurenine | Pseudomonas fluorescens | |
1.4.99.6 | 0.45 | - |
D-kynurenine | both methylene blue and 2,6-dichlorophenolindophenol specific enzymes | Pseudomonas fluorescens | |
1.4.99.6 | 0.47 | - |
D-tryptophan | both methylene blue and 2,6-dichlorophenolindophenol specific enzymes | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.99.6 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.99.6 | near homogeneity, two enzymes with different acceptor specificities, fractionation and chromatography techniques | Pseudomonas fluorescens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.99.6 | cell culture | one methylene blue specific, one 2,6-dichlorophenolindophenol specific enzyme, the methylene blue specific enzyme is only detectable in D-tryptophan grown cells | Pseudomonas fluorescens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.99.6 | 0.113 | - |
methylene blue specific enzyme | Pseudomonas fluorescens |
1.4.99.6 | 0.213 | - |
2,6-dichlorophenolindophenol specific enzyme | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.99.6 | D-alanine + H2O + 2,6-dichlorophenolindophenol | low activity | Pseudomonas fluorescens | pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-alanine + H2O + methylene blue | low activity | Pseudomonas fluorescens | pyruvate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-asparagine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-asparagine + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxosuccinamic acid + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-cysteine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-mercapto-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-cystine + H2O + methylene blue | - |
Pseudomonas fluorescens | ? + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-histidine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-histidine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-isoleucine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-isoleucine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-methyl-2-oxopentanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-kynurenine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-kynurenine + H2O + methylene blue | - |
Pseudomonas fluorescens | 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-leucine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-leucine + H2O + methylene blue | - |
Pseudomonas fluorescens | 4-methyl-2-oxopentanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-methionine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-norleucine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 2-oxohexanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-norleucine + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxohexanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-norvaline + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxopentanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-phenylalanine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-phenylalanine + H2O + methylene blue | - |
Pseudomonas fluorescens | phenylpyruvate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-serine + H2O + 2,6-dichlorophenolindophenol | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-serine + H2O + methylene blue | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-threonine + H2O + 2,6-dichlorophenolindophenol | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-threonine + H2O + methylene blue | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxobutanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-tryptophan + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-tryptophan + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-indole-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-tyrosine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-tyrosine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
1.4.99.6 | D-valine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-valine + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxoisopentanoate + NH3 + reduced methylene blue | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.99.6 | 7 | 8 | both methylene blue and 2,6-dichlorophenolindophenol specific enzymes | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.99.6 | FAD | - |
Pseudomonas fluorescens |