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Literature summary extracted from

  • Tamura, T.; Stadtman, T.C.
    A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity (1996), Proc. Natl. Acad. Sci. USA, 93, 1006-1011.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 3-Bromopropionate
-
 Homo sapiens
1.8.1.9 arsenite
-
 Homo sapiens
1.8.1.9 iodoacetate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0037
-
 thioredoxin thioredoxin from rat Homo sapiens
1.8.1.9 0.034
-
 thioredoxin thioredoxin from E. coli Homo sapiens
1.8.1.9 0.116
-
 DTNB
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.9 selenocysteine contains selenocysteine as part of the redox active selenosulfide in the active center Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 57000
-
 2 * 57000, SDS-PAGE Homo sapiens
1.8.1.9 110000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.1.9 additional information no glycosyl groups on the protein Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 lung adenocarcinoma cell line NCI-H441 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 31
-
 DTNB coupled assay, purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 GSSG + NADPH
-
 Homo sapiens GSH + NADP+
-
 ?
1.8.1.9 menadione + NADPH
-
 Homo sapiens ?
-
 ?
1.8.1.9 oxidized lipoate + NADPH
-
 Homo sapiens ?
-
 ?
1.8.1.9 rat thioredoxin + NADP+
-
 Homo sapiens rat thioredoxin disulfide + NADPH + H+
-
 r
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Homo sapiens thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Homo sapiens thioredoxin disulfide + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 57000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 1.83
-
 rat thioredoxin insulin coupled assay Homo sapiens
1.8.1.9 5.58
-
 thioredoxin insulin coupled assay method Homo sapiens
1.8.1.9 29.5
-
 DTNB
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.9 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Homo sapiens
1.8.1.9 NADPH
-
 Homo sapiens