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Literature summary extracted from
- Intersubunit interactions in Plasmodium falciparum thioredoxin reductase (2000), J. Biol. Chem., 275, 40874-40878.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | cysteine | 1 pair of redox active cysteines at the N-terminal active center and 1 pair of selenenylsulfide at the C-terminus, crucial for the reduction of thioredoxin | Plasmodium falciparum |  |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | medicine | target for a chemotherapeutic intervention of malaria | Plasmodium falciparum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | expression of His-tagged or Strep-tagged wild-type and mutants in Escherichia coli, DNA sequence analysis | Plasmodium falciparum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | C535S | construction of a homodimer and heterodimer, the latter containing 1 mutant and 1 wild-type subunit, activity is reduced by 56 and 92%, respectively | Plasmodium falciparum |
| 1.8.1.9 | C535S/C88A | double mutant, construction of a homodimer and a heterodimer, the latter containing 1 mutant and 1 wild-type subunit, activity is reduced by 89 and 95%, respectively | Plasmodium falciparum |
| 1.8.1.9 | C88A | construction of a homodimer and a heterodimer, the latter containing 1 mutant and 1 wild-type subunit, activity is reduced by 4 and 90% | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | thioredoxin + NADP+ | Plasmodium falciparum | detoxification of hydrogen peroxide, protection of the cell against oxidative damage | thioredoxin disulfide + NADPH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Plasmodium falciparum |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.1.9 | thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | catalytic mechanism | Plasmodium falciparum | |
| 1.8.1.9 | thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | oxidation-reduction cycle of thioredoxin | Plasmodium falciparum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | additional information | - |
quantification by DTNB coupled assay method | Plasmodium falciparum |
| 1.8.1.9 | 0.2 | - |
purified double mutant C535S/C88A as homodimer, insulin coupled assay | Plasmodium falciparum |
| 1.8.1.9 | 0.3 | - |
purified mutant C535S as homodimer, insulin coupled assay | Plasmodium falciparum |
| 1.8.1.9 | 0.4 | - |
homodimer of mutant C88A, insulin coupled assay | Plasmodium falciparum |
| 1.8.1.9 | 0.4 | - |
purified double mutant C535S/C88A as heterodimer with the wild-type, insulin coupled assay | Plasmodium falciparum |
| 1.8.1.9 | 1.5 | - |
purified mutant C535A as heterodimer with the wild-type, insulin coupled assay | Plasmodium falciparum |
| 1.8.1.9 | 2.2 | - |
purified mutant C88A as heterodimer with the wild-type, insulin coupled assay | Plasmodium falciparum |
| 1.8.1.9 | 3.9 | - |
purified wild-type enzyme, insulin coupled assay | Plasmodium falciparum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Plasmodium falciparum | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Plasmodium falciparum | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 1.8.1.9 | additional information | redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors | Plasmodium falciparum | ? | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | coupled assay with DTNB | Plasmodium falciparum | thioredoxin disulfide + NADPH | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Plasmodium falciparum | thioredoxin disulfide + NADPH | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | detoxification of hydrogen peroxide, protection of the cell against oxidative damage | Plasmodium falciparum | thioredoxin disulfide + NADPH | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | in presence of NADPH, coupled assay | Plasmodium falciparum | thioredoxin disulfide + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | dimer | - |
Plasmodium falciparum |
| 1.8.1.9 | More | intersubunit organisation | Plasmodium falciparum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | More | 2 classes of thioredoxin reductases: high molecular weight or large proteins e.g. in mammalia, Plasmodium falsiparum and Caenorhabditis elegans, and low molecular weight proteins e.g. in bacteria | Plasmodium falciparum |
| 1.8.1.9 | TrxT | - |
Plasmodium falciparum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Plasmodium falciparum | |
| 1.8.1.9 | NADPH | - |
Plasmodium falciparum | |
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