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Literature summary extracted from

  • Wang, P.F.; Veine, D.M.; Ahn, S.H.; Williams, C.H., Jr.
    A stable mixed disulfide between thioredoxin reductase and its substrate, thioredoxin: preparation and characterization (1996), Biochemistry, 35, 4812-4819.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 subunit complex of enzyme via C135 with thioredoxin C32, mutant complex S135-S32, expression from plasmid Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 C135S/C32S via the active disulfide centers a subunit complex of tightly bound enzyme, C135 and C138, and thioredoxin, C32 and C35, is formed, exchange of one cysteine for one serine in each protein by site-directed mutagenesis, conformation analysis Escherichia coli
1.8.1.9 C535S site-directed mutagenesis, changed conformation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 additional information
-
 additional information
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 recombinant enzyme-thioredoxin subunit complex C135-C32 and mutant S135-S32 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.9 thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ electron transfer in the enzyme complex of apoenzyme, FAD and thioredoxin with NADPH Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 additional information redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors Escherichia coli ?
-
 ?
1.8.1.9 thioredoxin + 3-acetylpyridine adenine dinucleotide wild-type enzyme, mutant enzyme C135S and thioredoxin in subunit complex C135-C32S with the enzyme Escherichia coli thioredoxin disulfide + reduced 3-acetylpyridine adenine dinucleotide
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Escherichia coli thioredoxin disulfide + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TrR
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 additional information
-
 additional information transhydrogenase activity of mutants and wild-type enzyme with thioredoxin and FAD Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 3-acetylpyridine adenine dinucleotide
-
 Escherichia coli
1.8.1.9 FAD
-
 Escherichia coli
1.8.1.9 NADPH
-
 Escherichia coli