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Literature summary extracted from

  • Lik-Shing Tsang, M.; Weatherbee, J.A.
    Thioredoxin, glutaredoxin, and thioredoxin reductase from cultured HeLa cells (1981), Proc. Natl. Acad. Sci. USA, 78, 7478-7482.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 5,5'-dithiobis(2-nitrobenzoate) above 0.1 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 58000
-
 2 * 58000, SDS-PAGE Homo sapiens
1.8.1.9 105000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Homo sapiens
-
 HeLa cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 HeLa cells, partially Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 42
-
 purified enzyme, coupled assay with DTNB Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay with adenosine 3'-phosphate 5'-phosphosulfate reductase Homo sapiens thioredoxin disulfide + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 58000, SDS-PAGE Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Homo sapiens
1.8.1.9 NADPH
-
 Homo sapiens