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Literature summary extracted from
- Rat liver thioredoxin and thioredoxin reductase: purification and characterization (1982), Biochemistry, 21, 6628-6633.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | cysteine | part of the active site | Rattus norvegicus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | arsenite | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 0.0025 | - |
thioredoxin | substrate: thioredoxin from calf and rat | Rattus norvegicus | |
| 1.8.1.9 | 0.0025 | - |
reductase | thioredoxin from rat, bovine substrate | Rattus norvegicus | |
| 1.8.1.9 | 0.006 | - |
NADPH | - |
Rattus norvegicus | |
| 1.8.1.9 | 0.045 | - |
thioredoxin | substrate: thioredoxin from E. coli | Rattus norvegicus | |
| 1.8.1.9 | 0.66 | - |
DTNB | - |
Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 58000 | - |
2 * 58000, SDS-PAGE | Rattus norvegicus |
| 1.8.1.9 | 116000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 35 | - |
purified enzyme, coupled assay with DTNB | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 1.8.1.9 | alloxan + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| 1.8.1.9 | L-cysteine + NADPH | - |
Rattus norvegicus | L-cystine + NADP+ | - |
? | |
| 1.8.1.9 | menadione + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| 1.8.1.9 | oxidized lipoamide + NADPH | - |
Rattus norvegicus | lipoamide disulfide + NADP+ | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | - |
Rattus norvegicus | thioredoxin disulfide + NADPH | - |
ir | |
| 1.8.1.9 | thioredoxin + NADP+ | - |
Rattus norvegicus | thioredoxin disulfide + NADPH + H+ | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | dimer | 2 * 58000, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 50 | - |
thioredoxin | - |
Rattus norvegicus | |
| 1.8.1.9 | 66.7 | - |
DTNB | - |
Rattus norvegicus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Rattus norvegicus | |
| 1.8.1.9 | NADPH | - |
Rattus norvegicus | |
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