BRENDA - Enzyme Database show

Purification and characterization of an inducible dissimilatory type sulfite reductase from Clostridium pasteurianum

Harrison, G.; Curle, C.; Laishley, E.J.; Arch. Microbiol. 138, 72-78 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.99.5
KCN
1 mM, complete inhibition
Clostridium pasteurianum
1.8.99.5
additional information
not inhibitory: iodoacetate
Clostridium pasteurianum
1.8.99.5
NaN3
1 mM, 18% inhibition
Clostridium pasteurianum
1.8.99.5
Tris/HCl
Tris buffer has an inhibitory effect. Approximately 60% less activity than with phosphate buffer is obtained at pH 7.5
Clostridium pasteurianum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.99.5
0.35
-
sulfite
pH 7.5, 37C
Clostridium pasteurianum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.99.5
13400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
1.8.99.5
28400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
1.8.99.5
83600
-
PAGE
Clostridium pasteurianum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.99.5
Clostridium pasteurianum
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.99.5
hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O
50% of the activity with sulfite
-
-
?
1.8.99.5
additional information
no substrates: S3O62-, S2O32-. Methyl viologen is as effective as ferredoxin in coupling the sulfite reductase with hydrogenase while benzyl viologen is only 16% as effective as ferredoxin. No activity is observed with NAD, NADP, FAD or FMN. Varying the substrate concentration [SO 2-] from 1 to 2.5 micromol affects the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S2- formed from 2.5:1 to 3.1:1
394898
Clostridium pasteurianum
?
-
-
-
-
1.8.99.5
nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O
25% of the activity with sulfite
-
-
?
1.8.99.5
SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+
-
394898
Clostridium pasteurianum
HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O
40% of the activity with sulfite
-
-
?
1.8.99.5
sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
394898
Clostridium pasteurianum
hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O
overall reaction
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.99.5
heterotetramer
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.99.5
7.5
-
phosphate buffer
Clostridium pasteurianum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.99.5
additional information
no activity is observed with NAD, NADP, FAD or FMN
Clostridium pasteurianum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.99.5
additional information
no activity is observed with NAD, NADP, FAD or FMN
Clostridium pasteurianum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.99.5
KCN
1 mM, complete inhibition
Clostridium pasteurianum
1.8.99.5
additional information
not inhibitory: iodoacetate
Clostridium pasteurianum
1.8.99.5
NaN3
1 mM, 18% inhibition
Clostridium pasteurianum
1.8.99.5
Tris/HCl
Tris buffer has an inhibitory effect. Approximately 60% less activity than with phosphate buffer is obtained at pH 7.5
Clostridium pasteurianum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.99.5
0.35
-
sulfite
pH 7.5, 37C
Clostridium pasteurianum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.99.5
13400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
1.8.99.5
28400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
1.8.99.5
83600
-
PAGE
Clostridium pasteurianum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.99.5
hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O
50% of the activity with sulfite
-
-
?
1.8.99.5
additional information
no substrates: S3O62-, S2O32-. Methyl viologen is as effective as ferredoxin in coupling the sulfite reductase with hydrogenase while benzyl viologen is only 16% as effective as ferredoxin. No activity is observed with NAD, NADP, FAD or FMN. Varying the substrate concentration [SO 2-] from 1 to 2.5 micromol affects the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S2- formed from 2.5:1 to 3.1:1
394898
Clostridium pasteurianum
?
-
-
-
-
1.8.99.5
nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O
25% of the activity with sulfite
-
-
?
1.8.99.5
SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+
-
394898
Clostridium pasteurianum
HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O
40% of the activity with sulfite
-
-
?
1.8.99.5
sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
394898
Clostridium pasteurianum
hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O
overall reaction
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.99.5
heterotetramer
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.99.5
7.5
-
phosphate buffer
Clostridium pasteurianum