Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, Z.; Villarta-Snow, R.L.; Lubrano, G.J.; Guilbault, G.G.
    Glutathione amperometric enzyme microsensor (1994), Anal. Lett., 27, 263-271.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.8.3.3 analysis development of a glutathione micro-enzyme sensor for amperometric detection in vivo, immobilization of enzyme on microelectrode tips Penicillium sp.

General Stability

EC Number General Stability Organism
1.8.3.3 after 36 measurements with enzyme immobilized on microelectrode sensor loss of 30% activity Penicillium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.3 1.7
-
 glutathione immobilized enzyme on sensor surface out of cellulose acetate Penicillium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.3.3 glutathione + O2 Penicillium sp. measurement of glutathione in vivo via microelectrodes with immobilized enzyme glutathione disulfide + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.3 Penicillium sp.
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.8.3.3 4°C, phosphate buffered saline, immobilized enzyme on microelectrode sensor, loss of 20% activity, 68 days Penicillium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.3 cysteine-glycine + O2 enzyme immobilized on microelectrode sensor Penicillium sp. ?
-
 ?
1.8.3.3 glutathione + O2 enzyme immobilized on microsensor Penicillium sp. glutathione disulfide + H2O2
-
 ?
1.8.3.3 glutathione + O2 measurement of glutathione in vivo via microelectrodes with immobilized enzyme Penicillium sp. glutathione disulfide + H2O2
-
 ?
1.8.3.3 S-methylglutathione + O2 enzyme immobilized on microelectrode sensor, low activity Penicillium sp. ?
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.3.3 3.8 11 enzyme immobilized on microsensor surface out of cellulose acetate Penicillium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.3 FAD flavoprotein Penicillium sp.