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Literature summary extracted from

  • Ostrowski, M.C.; Kistler, W.S.
    Properties of a flavoprotein sulfhydryl oxidase from rat seminal vesicle secretion (1980), Biochemistry, 19, 2639-2645.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.3 additional information EDTA has no effect on oxidation reaction Rattus norvegicus
1.8.3.3 thiols at high concentrations above 20 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.3 0.7
-
 dithiothreitol
-
 Rattus norvegicus
1.8.3.3 4.4
-
 glutathione
-
 Rattus norvegicus
1.8.3.3 9.1
-
 L-cysteine
-
 Rattus norvegicus
1.8.3.3 32
-
 2-mercaptoethanol
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.3 66000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.3 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.3.3 seminal vesicle
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.3 2-mercaptoethanol + O2
-
 Rattus norvegicus mercaptoethanol disulfide
-
 ?
1.8.3.3 dithiothreitol + O2
-
 Rattus norvegicus ?
-
 ?
1.8.3.3 glutathione + O2
-
 Rattus norvegicus glutathione disulfide + H2O2
-
 ?
1.8.3.3 L-cysteine + O2
-
 Rattus norvegicus L-cystine
-
 ?
1.8.3.3 additional information not: D,L-homocysteine Rattus norvegicus ?
-
 ?
1.8.3.3 reduced RNase A + O2
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.3.3 monomer 1 * 66000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.3 25
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.3.3 60
-
 3.5 min, 50% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.3 7
-
 substrate dithioerythritol Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.3.3 5 10 minimal activity below pH 5.0, active at least up to pH 10.0 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.3 FAD flavoprotein Rattus norvegicus
1.8.3.3 FAD 1 mol FAD per mol of enzyme Rattus norvegicus