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Literature summary extracted from

  • Roveri, A.; Maiorino, M.; Nisii, C.; Ursini, F.
    Purification and characterization of phospholipid hydroperoxide glutathione peroxidase from rat testis mitochondrial membranes (1994), Biochim. Biophys. Acta, 1208, 211-221.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.12 presence of thiol, e.g. 2-mercaptoethanol, during purification is necessary for stabilization Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.12 cytosol
-
Rattus norvegicus 5829
-
1.11.1.12 mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.12 Se
-
Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.12 22000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.12 glutathione + lipid hydroperoxide Rattus norvegicus
-
glutathione disulfide + lipid + H2O
-
?
1.11.1.12 additional information Rattus norvegicus controlled by gonadotropin ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.12 from cytosol and mitochondria Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.12 testis strongly linked to mitochondria of cells undergoing differentiation to spermatozoa, under gonadotropin control Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.12 additional information
-
specific activity of cytosolic and mitochondrial enzymes with several hydroperoxide substrates and reducing agents Rattus norvegicus
1.11.1.12 109900
-
purified enzyme from cytosol Rattus norvegicus
1.11.1.12 146300
-
purified enzyme from mitochondria Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.12 glutathione + cumene hydroperoxide
-
Rattus norvegicus ?
-
?
1.11.1.12 glutathione + H2O2
-
Rattus norvegicus ?
-
?
1.11.1.12 glutathione + L-alpha-phosphatidylcholine hydroperoxide
-
Rattus norvegicus ?
-
?
1.11.1.12 glutathione + LDL hydroperoxides
-
Rattus norvegicus ?
-
?
1.11.1.12 glutathione + lipid hydroperoxide
-
Rattus norvegicus glutathione disulfide + lipid + H2O
-
?
1.11.1.12 glutathione + lipid hydroperoxide dithiothreitol and dithioerythritol are 3fold more active than glutathione, while 2-mercaptoethanol and L-cysteine show 50% and 20% of the activity with glutathione as reductants, respectively Rattus norvegicus glutathione disulfide + lipid + H2O
-
?
1.11.1.12 glutathione + tert-butyl hydroperoxide
-
Rattus norvegicus ?
-
?
1.11.1.12 additional information controlled by gonadotropin Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.12 monomer 1 * 22000, SDS-PAGE Rattus norvegicus
1.11.1.12 More cytosolic enzyme shows also a minor component in SDS-PAGE at higher molecular weight, which is not further characterized Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.12 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.12 7.5
-
assay at Rattus norvegicus