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Literature summary extracted from

  • Chambers, S.J.; Lambert, N.; Williamson, G.
    Purification of a cytosolic enzyme from human liver with phospholipid hydroperoxide glutathione peroxidase activity (1994), Int. J. Biochem., 26, 1279-1286.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.12 deoxycholate in reaction with cumene hydroperoxide and linoleic acid hydroperoxide Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.12 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.12 Se
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.12 16100
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.12 glutathione + lipid hydroperoxide Homo sapiens protection of biomembranes against oxidative damage glutathione disulfide + lipid + H2O
-
 ?
1.11.1.12 additional information Homo sapiens enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes ?
-
 ?
1.11.1.12 additional information Homo sapiens enzyme inhibits peroxidation of microsomes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.12
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.12 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.12 0.009
-
-
 Homo sapiens
1.11.1.12 160
-
 purified enzyme, substrate linoleic acid hydroperoxide Homo sapiens
1.11.1.12 190
-
 purified enzyme, substrate cumene hydroperoxide Homo sapiens
1.11.1.12 210
-
 purified enzyme, substrate H2O2 Homo sapiens
1.11.1.12 336
-
 purified enzyme, substrate (beta-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatidylcholine Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.11.1.12 -40°C, about 30% loss of activity during 1 freeze-thaw-cycle Homo sapiens
1.11.1.12 4°C, loss of 10% activity in 1 week Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.12 glutathione + (beta-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatidylcholine
-
 Homo sapiens ?
-
 ?
1.11.1.12 glutathione + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-3-phosphatidylcholine
-
 Homo sapiens ?
-
 ?
1.11.1.12 glutathione + cumene hydroperoxide
-
 Homo sapiens ?
-
 ?
1.11.1.12 glutathione + H2O2
-
 Homo sapiens ?
-
 ?
1.11.1.12 glutathione + linoleic acid hydroperoxide
-
 Homo sapiens ?
-
 ?
1.11.1.12 glutathione + lipid hydroperoxide
-
 Homo sapiens glutathione disulfide + lipid + H2O
-
 ?
1.11.1.12 glutathione + lipid hydroperoxide protection of biomembranes against oxidative damage Homo sapiens glutathione disulfide + lipid + H2O
-
 ?
1.11.1.12 additional information enzyme inhibits lipid peroxidation, which is induced by Fe3+-triethylenetetramine complex, NADPH and ascorbate in presence of glutathione, therefore a physiological amount of alpha-tocopherol, vitamin E, is necessary in the membranes Homo sapiens ?
-
 ?
1.11.1.12 additional information enzyme inhibits peroxidation of microsomes Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.12 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.12 91
-
 (beta-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatidylcholine
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.12 7.6
-
 assay at Homo sapiens