Literature summary extracted from

Biaglow, J.E.; Donahue, J.; Tuttle, S.; Held, K.; Chrestensen, C.; Mieyal, J.
A method for measuring disulfide reduction by cultured mammalian cells: relative contributions of glutathione-dependent and glutathione-independent mechanisms (2000), Anal. Biochem., 281, 77-86.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.8.4.2	1,3-Bis(2-chloroethyl)-1-nitrosourea	-	Cricetulus griseus
1.8.4.2	1,3-Bis(2-chloroethyl)-1-nitrosourea	-	Homo sapiens
1.8.4.2	arsenite	-	Cricetulus griseus
1.8.4.2	arsenite	-	Homo sapiens
1.8.4.2	Phenylarsine oxide	-	Cricetulus griseus
1.8.4.2	Phenylarsine oxide	complete inhibition at 1 mM	Homo sapiens
1.8.4.2	selenium oxide	-	Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.8.4.2	GSH + protein disulfide	Homo sapiens	-	GSSG + protein-dithiol	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.4.2	Cricetulus griseus	-	chinese hamster	-
1.8.4.2	Homo sapiens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.8.4.2	insulinoma cell	-	Homo sapiens	-
1.8.4.2	lung	A549 carcinoma	Homo sapiens	-
1.8.4.2	mammary gland	MCF7 carcinoma	Homo sapiens	-
1.8.4.2	ovary	-	Cricetulus griseus	-
1.8.4.2	prostate gland	DU145 carcinoma	Homo sapiens	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.8.4.2	additional information	-	specific activities of different human carcinoma cell lines with different thiol and disulfide substrates	Homo sapiens
1.8.4.2	0.0035	-	A549 carcinoma cells, substrates GSH, cystamine or cystine	Homo sapiens
1.8.4.2	0.0075	-	A549 carcinoma cells, substrate dithiothreitol	Homo sapiens
1.8.4.2	0.03	-	A549 carcinoma cells, substrate hydroxyethyl disulfide	Homo sapiens
1.8.4.2	0.067	-	A549 carcinoma cells, substrate lipoate	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.8.4.2	alpha-lipoate + protein disulfide	-	Homo sapiens	?	-	?
1.8.4.2	cysteamine + protein disulfide	low activity	Homo sapiens	?	-	?
1.8.4.2	dithiothreitol + protein disulfide	low activity	Homo sapiens	?	-	?
1.8.4.2	GSH + protein disulfide	-	Homo sapiens	GSSG + protein-dithiol	-	?
1.8.4.2	hydroxyethyl disulfide + protein disulfide	-	Homo sapiens	?	-	?

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Release 2023.1 (January 2023)