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Literature summary extracted from

  • El-Waer, A.F.; Smith, K.; McKie, J.H.; Benson, T.; Fairlamb, A.H.; Douglas, K.T.
    The glutamyl binding site of trypanothione reductase from Crithidia fasciculata: enzyme kinetic properties of.gamma-glutamyl-modified substrate analogs (1993), Biochim. Biophys. Acta, 1203, 93-98.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Crithidia fasciculata
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma congolense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.12 0.052
-
 N-benzyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 0.225
-
 L-gamma-glutamyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 0.251
-
 tert-butyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 0.448
-
 4-aminobutyryl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 0.693
-
 L-beta-aspartyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 0.968
-
 L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.12 trypanothione disulfide + NADPH Crithidia fasciculata
-
 trypanothione + NADP+
-
 ?
1.8.1.12 trypanothione disulfide + NADPH Trypanosoma congolense
-
 trypanothione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.12 Crithidia fasciculata
-
-
-
1.8.1.12 Trypanosoma congolense
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.12 trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ neither the alpha-carboxy nor the alpha-amino function of the L-gamma-glutamyl group is essential for activity, can be replaced by uncharged benzyloxycarbonyl or tert-butyloxycarbonyl groups Crithidia fasciculata
a
1.8.1.12 trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ substrate binding structure, structural model Trypanosoma congolense
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.12 4-aminobutyryl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+ substrate analogue Crithidia fasciculata 2 N-(4-aminobutanoyl)-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
 ?
1.8.1.12 L-beta-aspartyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+ substrate analogue Crithidia fasciculata 2 L-beta-aspartyl-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
 ?
1.8.1.12 L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+ substrate analogue Crithidia fasciculata 2 L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
 ?
1.8.1.12 L-gamma-glutamyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+ substrate analogue Crithidia fasciculata 2 L-gamma-glutamyl-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
 ?
1.8.1.12 additional information glutaryl-L-cysteinylglycyl-3-methylaminopropylamide disulfide is no substrate Crithidia fasciculata ?
-
 ?
1.8.1.12 N-benzyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+ substrate analogue Crithidia fasciculata 2 N-[(benzyloxy)carbonyl]-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
 ?
1.8.1.12 tert-butyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+ substrate analogue Crithidia fasciculata 2 N-(tert-butoxycarbonyl)-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
 ?
1.8.1.12 trypanothione disulfide + NADPH
-
 Crithidia fasciculata trypanothione + NADP+
-
 ?
1.8.1.12 trypanothione disulfide + NADPH
-
 Trypanosoma congolense trypanothione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.12 TR
-
 Crithidia fasciculata
1.8.1.12 TR
-
 Trypanosoma congolense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.12 21.8
-
 4-aminobutyryl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 53.2
-
 L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 61.3
-
 L-beta-aspartyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 101
-
 tert-butyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 109
-
 N-benzyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata
1.8.1.12 818
-
 L-gamma-glutamyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide
-
 Crithidia fasciculata

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.12 FAD
-
 Crithidia fasciculata
1.8.1.12 FAD
-
 Trypanosoma congolense
1.8.1.12 NADPH
-
 Crithidia fasciculata
1.8.1.12 NADPH
-
 Trypanosoma congolense