Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cunningham, M.L.; Fairlamb, A.H.
    Trypanothione reductase from Leishmania donovani. Purification, characterization and inhibition by trivalent antimonials (1995), Eur. J. Biochem., 230, 460-468.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.12 additional information activity is highly dependent on ionic strength, optimum activity at 40 mM NaCl Leishmania donovani
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Leishmania donovani

Application

EC Number Application Comment Organism
1.8.1.12 medicine target for rational approach to design new anti-leishmanial chemotherapeutic agents Leishmania donovani

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.12 cloned into expression vector and expressed in wild-type cells Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.12 antimony chloride assay containing 10% vol. methanol Leishmania donovani
1.8.1.12 melarsen-trypanothione disulfide adduct i.e. adduct of p-(4,6-diamino-S-triazinyl-2-yl)aminophenyl arsenoxide with N1,N8-bis-(glutathionyl)spermidine Leishmania donovani
1.8.1.12 melarsen-trypanothione disulfide-adduct
-
Leishmania donovani
1.8.1.12 NaCl below 5% remaining activity at 1 M Leishmania donovani
1.8.1.12 potassium antimony tartrate assay containing 10% vol. methanol Leishmania donovani
1.8.1.12 triostam assay containing 10% vol. methanol Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.12 0.009
-
NADPH
-
Leishmania donovani
1.8.1.12 0.036
-
trypanothione disulfide
-
Leishmania donovani
1.8.1.12 0.115
-
glutathionylspermidine disulfide
-
Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.12 50900
-
2 * 50900, SDS-PAGE Leishmania donovani
1.8.1.12 106000
-
gel filtration Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.12 Leishmania donovani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.12 recombinant overexpression of the enzyme from plasmid Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.12 0.2
-
crude extract of wild-type cells Leishmania donovani
1.8.1.12 2.1
-
crude extract cells overexpressing the recombinant enzyme Leishmania donovani
1.8.1.12 112.7
-
purified enzyme Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.12 glutathionylspermidine disulfide + NADPH
-
Leishmania donovani 2-glutathionylspermidine + NADP+
-
?
1.8.1.12 trypanothione disulfide + NADPH trypanothione is the oxidized form of N1,N8-bis-(glutathionyl)spermidine, the latter form a stable adduct with melarsen oxide Leishmania donovani trypanothione + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.12 dimer 2 * 50900, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
1.8.1.12 TR
-
Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.12 179
-
trypanothione disulfide
-
Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.12 7.4
-
additional 2 minor optima at pH 6.6 and pH 8.2 Leishmania donovani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.12 6.5 9
-
Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.12 FAD
-
Leishmania donovani
1.8.1.12 NADPH
-
Leishmania donovani

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.12 0.009
-
melarsen-trypanothione disulfide-adduct
-
Leishmania donovani