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Literature summary extracted from

  • Davioud-Charvet, E.; Becker, K.; Landry, V.; Gromer, S.; Loge, C.; Sergheraert, C.
    Synthesis of 5,5'-dithiobis(2-nitrobenzamides) as alternative substrates for trypanothione reductase and thioredoxin reductase: a microtiter colorimetric assay for inhibitor screening (1999), Anal. Biochem., 268, 1-8.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma cruzi

Application

EC Number Application Comment Organism
1.8.1.12 medicine potential target for drug design against trypanosomiasis Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.12 overexpression in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.12 5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
1.8.1.12 clomipramine recombinant enzyme Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.12 0.035
-
 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
1.8.1.12 0.045
-
 trypanothione disulfide recombinant enzyme Trypanosoma cruzi
1.8.1.12 0.3
-
 5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.12 Trypanosoma cruzi
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.12 137
-
 recombinant purified enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.12 5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) + NADPH + H+
-
 Trypanosoma cruzi ?
-
 r
1.8.1.12 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) + NADPH + H+
-
 Trypanosoma cruzi ?
-
 ?
1.8.1.12 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) + NADPH + H+
-
 Trypanosoma cruzi ?
-
 r
1.8.1.12 additional information reduces 5,5'-dithiobis(2-nitrobenzamides) Trypanosoma cruzi ?
-
 ?
1.8.1.12 trypanothione disulfide + NADPH
-
 Trypanosoma cruzi trypanothione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.12 TR
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.12 25
-
 assay at Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.12 125
-
 5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
1.8.1.12 125
-
 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
1.8.1.12 240
-
 trypanothione disulfide recombinant enzyme Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.12 7.25
-
 assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.12 FAD
-
 Trypanosoma cruzi
1.8.1.12 NADPH
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.12 0.0086
-
 clomipramine recombinant enzyme, with 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) Trypanosoma cruzi