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Literature summary extracted from

  • McCallum, M.J.; Barrett, J.
    The purification and properties of glutathione reductase from the cestode Moniezia expansa (1995), Int. J. Biochem. Cell Biol., 27, 393-401.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.8.1.7 medicine potential target for chemotherapy Moniezia expansa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 1,3-Bis-(2-chloroethyl)-1-nitrosourea
-
 Moniezia expansa
1.8.1.7 2,4,6-Trinitrobenzenesulfonate
-
 Moniezia expansa
1.8.1.7 5-nitro-2-furoic acid
-
 Moniezia expansa
1.8.1.7 5-nitroindole
-
 Moniezia expansa
1.8.1.7 GSSG
-
 Moniezia expansa
1.8.1.7 Na+
-
 Moniezia expansa
1.8.1.7 Nitrofurantoin
-
 Moniezia expansa
1.8.1.7 nitrofurazone
-
 Moniezia expansa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.021
-
 NADPH
-
 Moniezia expansa
1.8.1.7 0.076
-
 GSSG
-
 Moniezia expansa
1.8.1.7 0.35
-
 NADH
-
 Moniezia expansa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.7 cytoplasm
-
 Moniezia expansa 5737
-
1.8.1.7 mitochondrion small amount Moniezia expansa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 63000
-
 2 * 63000, SDS-PAGE Moniezia expansa
1.8.1.7 114000
-
-
 Moniezia expansa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 GSSG + NADPH Moniezia expansa maintenance of glutathione/GSSG ratio is a protective mechanism for intracellular thiols during growth in atmospheric oxygen glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Moniezia expansa
-
 cestode
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Moniezia expansa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 5.03
-
 purified enzyme Moniezia expansa

Storage Stability

EC Number Storage Stability Organism
1.8.1.7 4°C, 50 mM potassium phosphate, pH 7.4, 50% loss of activity after 10 days Moniezia expansa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADH + H+
-
 Moniezia expansa glutathione + NAD+
-
 ?
1.8.1.7 GSSG + NADPH mixed disulfide between coenzyme A and glutathione Moniezia expansa glutathione + NADP+
-
 ?
1.8.1.7 GSSG + NADPH maintenance of glutathione/GSSG ratio is a protective mechanism for intracellular thiols during growth in atmospheric oxygen Moniezia expansa glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 63000, SDS-PAGE Moniezia expansa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 37
-
 assay at Moniezia expansa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 5
-
 NADH + GSSG Moniezia expansa
1.8.1.7 7.4
-
 NADPH + GSSG Moniezia expansa