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Literature summary extracted from

  • Chung, Y.C.; Hurlbert, R.E.
    Purification and properties of the glutathione reductase of Chromatium vinosum (1975), J. Bacteriol., 123, 203-211.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.8.1.7 repeated freezing and thawing, stable Allochromatium vinosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 ADP
-
 Allochromatium vinosum
1.8.1.7 AgNO3
-
 Allochromatium vinosum
1.8.1.7 AMP
-
 Allochromatium vinosum
1.8.1.7 ATP
-
 Allochromatium vinosum
1.8.1.7 BaCl2
-
 Allochromatium vinosum
1.8.1.7 Ca2+
-
 Allochromatium vinosum
1.8.1.7 Cl-
-
 Allochromatium vinosum
1.8.1.7 Cu2+
-
 Allochromatium vinosum
1.8.1.7 Fe(NO3)2
-
 Allochromatium vinosum
1.8.1.7 glutathione product inhibition Allochromatium vinosum
1.8.1.7 KI
-
 Allochromatium vinosum
1.8.1.7 Mg2+
-
 Allochromatium vinosum
1.8.1.7 Mn2+
-
 Allochromatium vinosum
1.8.1.7 N-ethylmaleimide
-
 Allochromatium vinosum
1.8.1.7 p-hydroxymercuribenzoate
-
 Allochromatium vinosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.00052
-
 FAD
-
 Allochromatium vinosum
1.8.1.7 0.6
-
 NADH
-
 Allochromatium vinosum
1.8.1.7 7
-
 GSSG
-
 Allochromatium vinosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.7 diphosphate activates Allochromatium vinosum
1.8.1.7 Na+ activates Allochromatium vinosum
1.8.1.7 NH4+ activates Allochromatium vinosum
1.8.1.7 phosphate activates Allochromatium vinosum
1.8.1.7 SO42- stimulates Allochromatium vinosum

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Allochromatium vinosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Allochromatium vinosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 28.4
-
-
 Allochromatium vinosum

Storage Stability

EC Number Storage Stability Organism
1.8.1.7 -20°C, 2 years Allochromatium vinosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADH + H+
-
 Allochromatium vinosum glutathione + NAD+
-
 ?
1.8.1.7 GSSG + NADPH no activity Allochromatium vinosum glutathione + NADP+
-
 ?
1.8.1.7 additional information oxidized lipoamide and oxidized lipoic acid Allochromatium vinosum ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.7 50
-
 5 min, 60% loss of activity Allochromatium vinosum
1.8.1.7 65
-
 5 min, complete loss of activity Allochromatium vinosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7
-
 NADH + GSSG Allochromatium vinosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD FAD enzyme Allochromatium vinosum
1.8.1.7 NADH specific for NADH Allochromatium vinosum
1.8.1.7 NADPH
-
 Allochromatium vinosum