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Literature summary extracted from
- Mouse-liver glutathione reductase. Purification, kinetics, and regulation (1979), Eur. J. Biochem., 98, 487-499.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.8.1.7 | complete inactivation after incubation with NADH, reversible by addition of GSSG | Mus musculus |
| 1.8.1.7 | complete inactivation after incubation with NADPH, reversible by addition of GSSG | Mus musculus |
| 1.8.1.7 | dichloroindophenol protects against thermal inactivation at 80°C | Mus musculus |
| 1.8.1.7 | FAD, no stabilization against thermal inactivation at 80°C | Mus musculus |
| 1.8.1.7 | glutathione protects against thermal inactivation at 80°C | Mus musculus |
| 1.8.1.7 | GSSG protects against thermal inactivation at 80°C | Mus musculus |
| 1.8.1.7 | NADP+, stabilizes against thermal inactivation at 80°C | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | glutathione | product inhibition | Mus musculus |  |
| 1.8.1.7 | NADH | at concentration above 0.3 mM | Mus musculus | |
| 1.8.1.7 | NADP+ | competitive against NADPH | Mus musculus | |
| 1.8.1.7 | NADPH | at concentration above 0.3 mM | Mus musculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.0059 | - |
NADPH | - |
Mus musculus | |
| 1.8.1.7 | 0.107 | - |
GSSG | - |
Mus musculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 55000 | - |
2 * 55000, SDS-PAGE | Mus musculus |
| 1.8.1.7 | 105000 | - |
gel filtration | Mus musculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Mus musculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | - |
Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 158 | - |
- |
Mus musculus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.8.1.7 | -70°C, pH 7.0, 1 mM EDTA, 1 mM dithiothreitol, 0.020 mM FAD, 5 mM NADP+ | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | GSSG + NADPH | - |
Mus musculus | glutathione + NADP+ | - |
? | |
| 1.8.1.7 | additional information | branched mechanism | Mus musculus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | dimer | 2 * 55000, SDS-PAGE | Mus musculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 37 | - |
8 h, stable | Mus musculus |
| 1.8.1.7 | 75 | - |
10 min, stable | Mus musculus |
| 1.8.1.7 | 80 | - |
NADP+, GSSG, glutathione and dichloroindophenol protect against thermal inactivation at 80°C | Mus musculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | NADPH | - |
Mus musculus | |
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